Research on Anti-Coxsackievirus B Activity of Konjac Oligo-Glucomannan Sulfate in Hela Cell Line

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Abstract:

Glucomannan extracted from konjac was sulfated (konjac oligo-glucomannan sulfate, OGMS) to show selective anti- coxsackievirus B activity in vitro using Hela cell line as the host cell of coxsackievirus B. OGMS straight inhibited the virus according to different groups and CVB-RNA Replication in Hela cell by RT-PCR. The studies indicated that OGMS mainly acted as blocking virus invading function and its IC50 was 0.1483 mg/mL determined by Reed-Muench method and CVB-RNA Replication was inhibited as well. All these indicated that OGMS had evident in vitro anti- coxsackievirus B activity.

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Prof. Zheng Yi Jiang and Prof. Yun Hae Kim

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417-423

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February 2013

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[1] Kimberlin D W, Coen D M, Biron K K, et a1.Molecular mechanisms of antiviral resistance. Antiviral Res, 2001, 26 (4) : 36-40.

[2] Masahiro Matsuda, Shiro Shigeta, and Koichi Okutani. Antiviral activities of Marine Pseudomonas Polysaccharides and Their oversulfated Derivatives. Marine Biotechnology, 2005, 7(1): 68-73.

DOI: 10.1007/pl00011753

[3] Luo HY, Yang X. Quantitative detection IAV-RNA in culture media of 2. 2. 15 cell. China Journal of Modern Medicine , 2005, 15(3): 383-387.

[4] Jin-Rong Zhao, Yu-Jie Bai, Qing-Hua Zhang, Yan Wan, Ding Li, Xiao-Jun Yan. Detection of hepatitis B virus DNA by real-time PCR using TaqMan-MGB probe technology. World J Gastroenterology, 2005, 11(4): 508-510.

DOI: 10.3748/wjg.v11.i4.508

[5] Mahmoud Huleihel, Vladimer Ishanu. Antiviral effect of red microalgal polysaccharides on Herpes simplex and Varicella zoster viruses. Journal of Applied Phycology, 2001, 13: 127-134.

[6] Laura B, Talarico, Elsa B Damonte. Interference in dengue virus adsorption and uncoating by carrageeenans. Virology, 2007, 3(363): 473-485.

DOI: 10.1016/j.virol.2007.01.043

[7] Miaoa B, Genga M, Jing Lia, et a1.Sulfated polymannuroguluronate, a novel anti-acquired immune deficiency syndrome (AIDS) drug candidate, targeting CD4 in lymphocytes. Biochemical Pharmacology, 2004, 68: 641-649.

DOI: 10.1016/j.bcp.2004.04.009

[8] Chaidedgumjom A, Toyoda H, Woo ER et a1.Effect of(1→3)-and(1→4)-linkages of fully sulfated polysaccharides on their anti-coagulant activity.Carbohydrate Research, 2002, 337(10): 925-933.

[9] Mei Zhang, Peter C K Cheung, Lina Zhang. Evaluation of sulfated fungal b-glucans from the sclerotium of Pleurotus tuber-regium as a potential water-soluble anti-viral agent. Carbohydrate Research, 2004, 7(339): 2297-2301.

DOI: 10.1016/j.carres.2004.07.003

[10] BeressA , Wassermann O , Tahhan S , et a1.A new procedure for the isolation of anti-HIV compound (polysaccharides and polyphenols) from the marine alga Fucus Vesiculosus. J Nat Prod , 2003, 56(4): 478-482.

DOI: 10.1021/np50094a005

[11] Joung Han Yim, Sung Jin Kim, Se Hun Ahn, et a1. Antivirus effect of sulfated exopolysaccharide from the maine microalga Gyrodinium impudium strain KG03.Marin Biotechnology, 2004, 6(1): 7-25.

DOI: 10.1007/s10126-003-0002-z

[12] Talarico L B, Zibetti R G M, Fafia P C S, et a1.Anti-herpes simplex virus activity of sulfated galactans from the red seaweeds Gymnogongrus griffithsiae and Cryptonemia crenulata.Intern J of BioMacrom, 2004, 34: 63-71.

DOI: 10.1016/j.ijbiomac.2004.03.002

[13] Partha Ghosh, Utpal Adhikari, Prodyot K Ghosal, et a1.In vitro anti-herpetic activity of sulfated polysaccharide fractions from Caulerpa racemosa.Phytochemistry, 2004, 65: 3151-3l57.

DOI: 10.1016/j.phytochem.2004.07.025

[14] W Zhu, L C M Chiu, P K S Chan. Isolation and characterization of an anti-HSV polysaccharide from Prunella vulgaris. Phytomedicine, 2006, 11(24): 695-701.

[15] Yongwen Zhang, Paul Pui-Hay But, Hong-Xi Xu, Gillian D Delaney, Spencer H S Lee, Song F Lee. Chemical properties, mode of action, and in vivo anti-herpes activities of a lignin-carbohydrate complex from Prunella vulgaris. Antiviral research, 2007, 3(75): 242-249.

DOI: 10.1016/j.antiviral.2007.03.010