Corrosion is the destructive effect of environment on metals and alloys. Corrosion is a spontaneous phenomenon which causes metal conversion from zero oxidation degree to a species with high oxidation degree. As a result of this chemical interaction due to the corrosion phenomenon, irretrievable damages are made to the equipment and installations of various industries. Biocorrosion has occurred in the water supply pipes at Sarcheshmeh copper mine(Located in South of Iran), and in order to assess the amount of biocorrosion and remove these biological interactions we decided to chemically evaluate the water sample in the water supply pipelines at this mine. In this look, the water sample exist in the water supply pipelines at Sarcheshmeh copper mine was studied, sulfur corrosive bacteria were separated and identified and their biochemical activity was researched and studied and it was found that the target species are in fact Sulfolobus bacteria which have different enzymatic activities. Due to these enzymatic activities, a considerable amount of sulfur is accumulated on the cell surface. Based on various incubation environments, as a result of sulfur accumulation, Sulfolubos bacteria were identified.