ATP Measurements in Iron-Oxidizing Acidithiobacillus Ferrooxidans


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We applied a kit for bioluminescent ATP measurement based on the luciferase reaction to Acidithiobacillus ferrooxidans cultures. Determination limit for monitoring cell growth in the culture was 1.1 × 106 cells per ml. Determination errors varied between 1.5 and 10 % (RSD). ATP formation correlated with cell growth and iron oxidation up to limitation by iron(II). A maximum ATP content was approximately 1 amol per cell under the growth yield on iron of 1 × 1012 cells per mol Fe(II).



Advanced Materials Research (Volumes 71-73)

Edited by:

Edgardo R. Donati, Marisa R. Viera, Eduardo L. Tavani, María A. Giaveno, Teresa L. Lavalle, Patricia A. Chiacchiarini




M. Mandl et al., "ATP Measurements in Iron-Oxidizing Acidithiobacillus Ferrooxidans", Advanced Materials Research, Vols. 71-73, pp. 275-278, 2009

Online since:

May 2009




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