ATP Measurements in Iron-Oxidizing Acidithiobacillus Ferrooxidans
We applied a kit for bioluminescent ATP measurement based on the luciferase reaction to Acidithiobacillus ferrooxidans cultures. Determination limit for monitoring cell growth in the culture was 1.1 × 106 cells per ml. Determination errors varied between 1.5 and 10 % (RSD). ATP formation correlated with cell growth and iron oxidation up to limitation by iron(II). A maximum ATP content was approximately 1 amol per cell under the growth yield on iron of 1 × 1012 cells per mol Fe(II).
Edgardo R. Donati, Marisa R. Viera, Eduardo L. Tavani, María A. Giaveno, Teresa L. Lavalle, Patricia A. Chiacchiarini
M. Mandl et al., "ATP Measurements in Iron-Oxidizing Acidithiobacillus Ferrooxidans", Advanced Materials Research, Vols. 71-73, pp. 275-278, 2009