ATP Measurements in Iron-Oxidizing Acidithiobacillus Ferrooxidans

Abstract:

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We applied a kit for bioluminescent ATP measurement based on the luciferase reaction to Acidithiobacillus ferrooxidans cultures. Determination limit for monitoring cell growth in the culture was 1.1 × 106 cells per ml. Determination errors varied between 1.5 and 10 % (RSD). ATP formation correlated with cell growth and iron oxidation up to limitation by iron(II). A maximum ATP content was approximately 1 amol per cell under the growth yield on iron of 1 × 1012 cells per mol Fe(II).

Info:

Periodical:

Advanced Materials Research (Volumes 71-73)

Edited by:

Edgardo R. Donati, Marisa R. Viera, Eduardo L. Tavani, María A. Giaveno, Teresa L. Lavalle, Patricia A. Chiacchiarini

Pages:

275-278

DOI:

10.4028/www.scientific.net/AMR.71-73.275

Citation:

M. Mandl et al., "ATP Measurements in Iron-Oxidizing Acidithiobacillus Ferrooxidans", Advanced Materials Research, Vols. 71-73, pp. 275-278, 2009

Online since:

May 2009

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Price:

$35.00

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