ATP Measurements in Iron-Oxidizing Acidithiobacillus Ferrooxidans

Mandl, Martin; Markova, Romana; Lojek, A.

doi:10.4028/www.scientific.net/AMR.71-73.275

Paper Titles

Kinetics of Microbial Ferrous-Iron Oxidation by Leptospirillum Ferriphilum: Effect of Ferric-Iron on Biomass Growth
p.259

Experimental Study of Ferrous Iron Biooxidation by Leptospirillum Ferriphilum in Different Biofilm Reactors
p.263

Effect of the Energy and Carbon Source Limitations and Ferric Inhibition on Metabolic Parameters of Leptospirillum Ferrooxidans Growing in Chemostat
p.267

Ferrous Iron and Pyrite Oxidation by “Acidithiomicrobium” Species
p.271

ATP Measurements in Iron-Oxidizing Acidithiobacillus Ferrooxidans
p.275

Copper Resistance Mechanisms of Biomining Bacteria and Archaea Living under Extremely High Concentrations of Metals
p.279

The Characterization of Salt Tolerance in Biomining Microorganisms and the Search for Novel Salt Tolerant Strains
p.283

Interrelation between Cells and Extracellular Polymeric Substances (EPS) from Acidiphilium 3.2Sup(5) on Carbon Surfaces
p.287

Reductive Action of Activated Carbon on Ferric Iron Interferes on the Determination of the Oxidative Activity of Acidithiobacillus Ferrooxidans on Ferrous Iron
p.291

Home Advanced Materials Research Biohydrometallurgy 2009ATP Measurements in Iron-Oxidizing...

ATP Measurements in Iron-Oxidizing Acidithiobacillus Ferrooxidans

Abstract:

We applied a kit for bioluminescent ATP measurement based on the luciferase reaction to Acidithiobacillus ferrooxidans cultures. Determination limit for monitoring cell growth in the culture was 1.1 × 106 cells per ml. Determination errors varied between 1.5 and 10 % (RSD). ATP formation correlated with cell growth and iron oxidation up to limitation by iron(II). A maximum ATP content was approximately 1 amol per cell under the growth yield on iron of 1 × 1012 cells per mol Fe(II).

Info:

Periodical:

Advanced Materials Research (Volumes 71-73)

Main Theme:

Biohydrometallurgy 2009

Edited by:

Edgardo R. Donati, Marisa R. Viera, Eduardo L. Tavani, María A. Giaveno, Teresa L. Lavalle, Patricia A. Chiacchiarini

Pages:

275-278

DOI:

10.4028/www.scientific.net/AMR.71-73.275

Citation:

M. Mandl et al., "ATP Measurements in Iron-Oxidizing Acidithiobacillus Ferrooxidans", Advanced Materials Research, Vols. 71-73, pp. 275-278, 2009

Online since:

May 2009

Authors:

Martin Mandl, Romana Markova, A. Lojek

Keywords:

Acidithiobacillus ferrooxidans, ATP, Iron Oxidizing Bacteria

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