Attachment Behavior of Leaching Bacteria to Metal Sulfides Elucidated by Combined Atomic Force and Epifluorescence Microscopy

Abstract:

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The aim of the study was to quantify and to visualize colonization of metal sulfides by pure and mixed cultures. Strains of the genera Acidithiobacillus and Leptospirillum were tested. Sessile and planktonic cells were visualized by fluorescence microscopy using 4',6-diamidino-2-phenylindole (DAPI) and FISH. Additionally, atomic force microscopy was used for the investigations on cell morphology, spatial arrangement of cells on metal sulfides and mineral surface topography. It was shown that the morphology of sessile cells was totally different as compared with planktonic ones. Interactions of different species resulted in increased production of extracellular polymeric substances (EPS) or caused negligible-attaching bacteria to be incorporated into a biofilm by the good attaching ones. Consequently, biofilm formation was furthered.

Info:

Periodical:

Advanced Materials Research (Volumes 71-73)

Edited by:

Edgardo R. Donati, Marisa R. Viera, Eduardo L. Tavani, María A. Giaveno, Teresa L. Lavalle, Patricia A. Chiacchiarini

Pages:

337-340

DOI:

10.4028/www.scientific.net/AMR.71-73.337

Citation:

B. M. Florian et al., "Attachment Behavior of Leaching Bacteria to Metal Sulfides Elucidated by Combined Atomic Force and Epifluorescence Microscopy", Advanced Materials Research, Vols. 71-73, pp. 337-340, 2009

Online since:

May 2009

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Price:

$35.00

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