Visualisation of Bacterial Behaviour Using Tapping-Mode Atomic Force Microscopy
Ex-situ and in-situ Tapping Mode AFM were used to investigate responses of attached bacteria to stressful conditions. For ex-situ measurements, the AFM was equipped with a customised re-positioning stage and sample mount to permit re-examination of the same surface area. For in-situ measurements, the inoculated pyrite coupon was immersed in solution in a flow through cell. Initial experiments using Sulfobacillus thermosulfidooxidans indicated that increased acidity promoted EPS production but increased salinity resulted in cell detachment.
Edgardo R. Donati, Marisa R. Viera, Eduardo L. Tavani, María A. Giaveno, Teresa L. Lavalle, Patricia A. Chiacchiarini
N. Gorham et al., "Visualisation of Bacterial Behaviour Using Tapping-Mode Atomic Force Microscopy", Advanced Materials Research, Vols. 71-73, pp. 341-344, 2009