A Study of Biofilm and Non-Line-of-Sight Bio-Hydroxyapatite Coatings Using a Serratia sp.
This study describes biofilm formation as a non line-of-sight coating method on support materials such as polyurethane foam, porous glass, polypropylene (PP) and titanium alloy, using a Serratia sp., which can manufacture extracellular nanoscale scaffolded hydroxyapatite (HA) crystals via enzymatic cleavage of glycerol 2-phosphate (G2P) in the presence of CaCl2. Various microscopies and non-invasive magnetic resonance imaging were used to visualize the biofilm coating on the support surface. A novel micromanipulation technique was used to estimate the adhesive strength of native and HA-mineralized biofilms. The biofilm with HA was up to forty times stronger than that without HA. A coating of nano-HA (> 80 m) onto a biofilm-Ti disc was achieved.
Edgardo R. Donati, Marisa R. Viera, Eduardo L. Tavani, María A. Giaveno, Teresa L. Lavalle, Patricia A. Chiacchiarini
P. Yong et al., "A Study of Biofilm and Non-Line-of-Sight Bio-Hydroxyapatite Coatings Using a Serratia sp.", Advanced Materials Research, Vols. 71-73, pp. 741-744, 2009