A Study of Biofilm and Non-Line-of-Sight Bio-Hydroxyapatite Coatings Using a Serratia sp.


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This study describes biofilm formation as a non line-of-sight coating method on support materials such as polyurethane foam, porous glass, polypropylene (PP) and titanium alloy, using a Serratia sp., which can manufacture extracellular nanoscale scaffolded hydroxyapatite (HA) crystals via enzymatic cleavage of glycerol 2-phosphate (G2P) in the presence of CaCl2. Various microscopies and non-invasive magnetic resonance imaging were used to visualize the biofilm coating on the support surface. A novel micromanipulation technique was used to estimate the adhesive strength of native and HA-mineralized biofilms. The biofilm with HA was up to forty times stronger than that without HA. A coating of nano-HA (> 80 m) onto a biofilm-Ti disc was achieved.



Advanced Materials Research (Volumes 71-73)

Edited by:

Edgardo R. Donati, Marisa R. Viera, Eduardo L. Tavani, María A. Giaveno, Teresa L. Lavalle, Patricia A. Chiacchiarini






P. Yong et al., "A Study of Biofilm and Non-Line-of-Sight Bio-Hydroxyapatite Coatings Using a Serratia sp.", Advanced Materials Research, Vols. 71-73, pp. 741-744, 2009

Online since:

May 2009




[1] A.B. Cunningham, E.J. Bouwer and W. Characklis: Biofilms (John Wiley & Sons, New York 1990).

[2] T. R Garrett, M. Bhakoo and Z. Zhang: Prog. Nat. Sci. Vol. 18 (2008), p.1049.

[3] L.E. Macaskie, K.M. Bonthrone, P. Yong and D.T. Goddard: Microbiology. Vol. 146 (2000), p.1855.

[4] P. Pattanapipitpaisal, A.N. Mabbett, J.A. Finlay, A.J. Beswick, M. Paterson-Beedle, A. Essa, J. Wright, M.R. Tolley, U. Badar, N. Ahmed, J.L. Hobman, N.L. Brown, and L.E. Macaskie: Environ. Technol. Vol. 23 (2002), p.731.

DOI: 10.1080/09593332308618367

[5] L.E. Macaskie, P. Yong, M. Paterson-Beedle, A. Thackray, P.M. Marquis, R.L. Sammons, K. P Nott and L.D. Hall: J. Biotech. Vol. 118 (2005), p.187.

[6] P Yong, L.E. Macaskie, R.L. Sammons and P.M. Marquis: Biotech. Lett. Vol. 26 (2004), p.1723.

[7] M.J. Chen, Z. Zhang and T.R. Bott: Colloids & Surfaces B: Biointerfaces. Vol. 43 (2005), p.61.

[8] L. Clapham, R.J.C. Mclean, J.C. Nickel, J. Downey and J.W. Costerton: J Crystal Growth Vol. 104 (1990), p.475.

[9] D. Stickler, N. Morris, M.C. Moreno and N. Sabbuba: Eur. J. Clin. Microbiol. Infect. Dis. Vol. 17 (1998).

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