Cloud Point Extraction and Spectrophotometry Determination of Lead in Environment Water Using 5-Br-PADAP


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A simple, sensitive, green and low cost detection method based on the cloud point extraction (CPE) separation and spectrophotometry was proposed for the determination of lead. In pH=9.0 H3BO3 buffer solution, Pb(II) reacts with 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (5-Br-PADAP) in the presence of Triton X-100 yielding a hydrophobic complex, which then is extracted into micro-volume surfactant-rich phase. The calibration graph was linear in the range of 20-400 µg/L (at 560 nm). Under the optimized conditions, the detection limits of 10.94 µg/L and the relative standard deviations(RSD) of 2.0% (n=5) for Lead(II) were found, respectively. The sensitivity and absorbance of this method are at least five times higher when compared with that of usual 5-Br-PADAP spectrophotometry without CPE, and the proposed method has been applied to the determination of Lead in environment water samples with satisfactory results.



Edited by:

Helen Zhang, David Jin and X.J. Zhao




L. Gao et al., "Cloud Point Extraction and Spectrophotometry Determination of Lead in Environment Water Using 5-Br-PADAP", Advanced Materials Research, Vol. 830, pp. 345-348, 2014

Online since:

October 2013




[1] W. Guo, W. Liu, S. Jin, S. Hu, H. Zhang, Y. Peng: International Conference on Bioinformatics and Biomedical Engineering - ICBBE (2008), p.4504.

[2] Shemirani, Farzaneh , Kozani, R. Rahnama , Jamali, M. Reza , Assadi, Yaghoub and Hosseini, M. Milani: International Journal of Environmental Analytical Chemistry Vol. 14(2006), p.1105.


[3] H. Filik, D. Giray: Food Chemistry Vol. 130 (2012), p.209.

[4] A. Safavi, H. Abdollahi, M.R.H. Nezhad, R. Kamali: Spectrochimica Acta Part A Vol. 60 (2004), p.2897.

[5] Salmanipour, Ashraf, Taher, M. Ali: Journal of Solid State Electrochemistry Vol. 15(2011), p.2695.

[6] N. Şatıroğlu, Ç. Arpa: Microchimica Acta, Vol. 162(2008), p.107.

[7] M.M. Hassanien, M.H. Abdel-Rhman and A.A. El-Asmy: Transition Metal Chemistry Vol. 32 (2007), p.1025.

[8] H.I. Ulusoy, R. Gurkan, S. Ulusoy: Talanta Vol. 88 (2012), p.516.