Optimization of Extraction Process of Water-Soluble Polysaccharides from Porphyra by Response Surface Methodology
Polysaccharide from Porphyra is a kind of important biological active substance. Therefore，it is significant to effectively develop and utilize the polysaccharide from porphyra and improve the economic and social benefits of porphyra. In this paper, polysaccharide was extracted by the method of hot water immersed extraction and ethanol precipitation. Extraction process of water-soluble polysaccharides from Porphyra was optimized by response surface methodology. The content of polysaccharide was determined by sulfuric acid to phenol method. The results show that the extraction time is 4.4 h, extraction temperature is 88°Cand the ratio of material to liquid is1:42(g/mL). Polysaccharide is precipitated with 5 folds volume of ethanol for 6 h. The proportion of the extraction of the porphyra polysaccharide under this industrial process conditions is 10.012%.
Hui Li, Qunjie Xu and Honghua Ge
Y. C. Li et al., "Optimization of Extraction Process of Water-Soluble Polysaccharides from Porphyra by Response Surface Methodology", Advanced Materials Research, Vols. 864-867, pp. 526-530, 2014