In Vitro Evaluation of Candida albicans Biofilm Formation on Denture Base PMMA Resin Incorporated with Silver Nanoparticles and its Effect on Flexural Strength


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The aim of this study is to investigate the candidal biofilm formation on the silver nanoparticles (AgNPs) incorporated denture base heat-polymerized (poly methyl methacrylate), PMMA, resinand its flexural strength.Materials and methods:A total of 36PMMA resin specimens (15×15×2 mm3) were fabricated and divided into 4 groups based on their AgNPs contents (0%,0.5%, 1% and 1.5% w/w of polymer). The biofilm of clinical and reference strainsof C.albicanswere grown on PMMA resin specimens in the presence of yeast nitrogen base broth supplemented with 100 mM glucose at 37oC for 48 h and evaluated by tetrazolium reduction assay. The flexural strength of PMMA resin specimens (65×10×3.3 mm3) were tested by using three-point bending test. Data were analyzed by Kruskal-Wallis and Mann-Whitney U test at p<0.05. result:all="" 3="" groups="" with="" agnps="" showed="" significant="" reduction="" in="" biofilm="" formation="" of="" both="" strains="" i="">C.albicans compared to control (0% AgNPs) (p<0.05). Decreased flexural strength was observed with AgNPs groups compared with the control but within acceptable limit of ISO 20795-1. Conclusion:The reduction of the C. albicans biofilm was observed on the heat-polymerized PMMA resin incorporated with AgNPs. Regarding the flexural strength, the values were within ISO limit. These modified PMMA resin can be developed for the prevention or treatment of the candidal infection associated with the denture base material.



Edited by:

Brendan Gan, Y. Gan and Y. Yu




S. Bajracharya et al., "In Vitro Evaluation of Candida albicans Biofilm Formation on Denture Base PMMA Resin Incorporated with Silver Nanoparticles and its Effect on Flexural Strength", Advanced Materials Research, Vol. 905, pp. 51-55, 2014

Online since:

April 2014




* - Corresponding Author

[1] M.P. Patel, A.T. Cruchley, D.C. Coleman, H. Swai, M. Braden and D.M. Williams: BiomaterialsVol. 22(7) (2001), pp.2319-2324.

[2] A.N. Ellepola and L.P. Samaranayake: Crit Rev Oral Biol Med Vol. 11(2) (2000), pp.172-198.

[3] G. Ramage, E. Mowat, B. Jones, C. Williams andJ. Lopez-Ribot: Crit Rev Microbiol Vol. 35(4) (2009), pp.340-355.

[4] G.S. Baillie andL.J. Douglas: J Antimicrob Chemother Vol. 46 (2000), pp.397-403.

[5] Q.L. Feng, J. Wu, G.Q. Chen, F.Z. Cui, Kim TN and Kim JO: J Biomed Mater Res Vol. 52(4) (2000), pp.662-668.

[6] Y. Li, P. Leung, L. Yao, Q. W Song and E. Newton: J Hosp Infect Vol. 62 (2006), pp.58-63.

[7] U. Samuel and J.P. Guggenbichler: Int J Antimicrob Agents Vol. 23 (2004), pp.75-78.

[8] L.A. Casemiro, C.H. Gomes Martins, Fde.C. Piresode-Souza and H. Panzeri: Gerodontology Vol. 25(3) (2008), pp.187-194.

[9] M.Z. Kassaee, A. Akhavan, N. Sheikh and A. Sodager: J Appl Polymer Sci Vol. 110(2008), pp.1699-1703.

[10] J.D. Oei, Zhao WW, L. Chu, M.N. DeSilva, A. Ghimire, R.H. Rawls and K. Whang: Soc Biomater Vol. 100B (2012), pp.409-415.

[11] D.R. Monteiro, L. F Gorup, A.S. Takamiya, E.R. de Camargo, A.C. Filho and D.B. Barbosa: J Prosthodont Vol. 21(1) (2012), pp.7-15.

[12] A.F. Wady, A.L. Machado, V. Zucolotto, C.A. Zamperini, E. Berni and C.E. Vergani: J Appl Microbiol Vol. 112 (2012), pp.1163-1172.

[13] M.S. Beyli and J.A. von Fraunhofer: J Prosthet Dent Vol. 46 (3) (1981), pp.238-241.

[14] Y. Jin, L.P. Samaranayake, Y. Samaranayake and H.K. Yip: Arch Oral Biol Vol. 49 (2004), pp.789-798.

[15] The International Standard. ISO 20795-1: 2008 (E). Dentistry-Base polymers-Part 1: denture base polymers.

[16] C. Salerno, M. Pascale, M. Contaldo, V. Esposite, M. Busciolano, L. Mililo et al: Med Oral Ptol Oral Cor Buccal Vol. 16 (2) (2011), pp.139-143.

[17] M.V. Martin: J Med Vet Mycol Vol. 27 (1989), pp.63-70.

[18] S.P. Hawser and L. Douglas: Antimicrob Chemother Vol. 39 (1995), pp.2128-2131.

[19] W.K. Jung, H.C. Koo, K.W. Kim, S. Shin, S.H. Kim and Y.H. Park: Appl Environ Microbiol Vol. 74 (4) (2008), pp.2171-2178.

[20] K.J. Kim, W.S. Sung, B.K. Suh, S.K. Moon, J. S Choi, J.G. Kim and D. G Lee: Biometals Vol. 22 (2009), pp.235-242.

[21] I.S. Hwang, J. Lee, J.H. Hwang, K. J Kim and D. G Lee: FEBS Journal Vol. 279 (2012), pp.1327-1338.

[22] A. Akhavan, N. Sheikh and R. Beteshobabrud: J Nuclear Sci. Tech. Vol. 50 (2010), pp.80-84.

[23] S.J. Ahn, S.J. Lee, J.K. Kook and B.S. Lim: Dent Mater Vol. 25 (2) (2009), pp.206-213.

[24] T. Shibata, N. Hamada, K. Kimoto, T. Sawada, T. Sawada, K. Hidefumi et al.: Dent Mater J Vol. 26(3) (2007), pp.437-444.

[25] A. Sodager, M. Z Kassaee, A. Akhavan, N. Javadi, S. Arab and M.J. Khaeazifard: J Prosthodont Res Vol. 56 (2012), pp.120-124.