In Vitro Evaluation of Candida albicans Biofilm Formation on Denture Base PMMA Resin Incorporated with Silver Nanoparticles and its Effect on Flexural Strength
The aim of this study is to investigate the candidal biofilm formation on the silver nanoparticles (AgNPs) incorporated denture base heat-polymerized (poly methyl methacrylate), PMMA, resinand its flexural strength.Materials and methods:A total of 36PMMA resin specimens (15×15×2 mm3) were fabricated and divided into 4 groups based on their AgNPs contents (0%,0.5%, 1% and 1.5% w/w of polymer). The biofilm of clinical and reference strainsof C.albicanswere grown on PMMA resin specimens in the presence of yeast nitrogen base broth supplemented with 100 mM glucose at 37oC for 48 h and evaluated by tetrazolium reduction assay. The flexural strength of PMMA resin specimens (65×10×3.3 mm3) were tested by using three-point bending test. Data were analyzed by Kruskal-Wallis and Mann-Whitney U test at p<0.05. result:all="" 3="" groups="" with="" agnps="" showed="" significant="" reduction="" in="" biofilm="" formation="" of="" both="" strains="" i="">C.albicans compared to control (0% AgNPs) (p<0.05). Decreased flexural strength was observed with AgNPs groups compared with the control but within acceptable limit of ISO 20795-1. Conclusion:The reduction of the C. albicans biofilm was observed on the heat-polymerized PMMA resin incorporated with AgNPs. Regarding the flexural strength, the values were within ISO limit. These modified PMMA resin can be developed for the prevention or treatment of the candidal infection associated with the denture base material.
Brendan Gan, Y. Gan and Y. Yu
S. Bajracharya et al., "In Vitro Evaluation of Candida albicans Biofilm Formation on Denture Base PMMA Resin Incorporated with Silver Nanoparticles and its Effect on Flexural Strength", Advanced Materials Research, Vol. 905, pp. 51-55, 2014