Novel Nitrogen Rich Polymers and Chitosan for Tissue Engineering of Intervertebral Discs

Abstract:

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Degenerative disc disease has been implicated as a major component of spine pathology. However, though biological repair of the degenerate disc would be the ideal treatment, there is a lack of a universally accepted scaffold for tissue engineering of intervertebral discs (IVD) and little is known of how to differentiate mesenchymal stem cells (MSCs) to a disc-like phenotype. We show that 2.5% Protasan® UP G213 cross-linked to 5% genipin might be a promising scaffold for disc tissue engineering. Furthermore, we have developed extremely N-rich plasma polymer layers, which we call "PPE:N" (N-doped plasma-polymerized ethylene, containing up to 36% [N]). We show that PPE:N almost completely suppresses the expression not only of type X collagen, but also of osteogenic marker genes such as alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteocalcin (OC). In contrast, neither aggrecan nor types 1 collagen expression were significantly affected. These results indicate that PPE:N coatings may be suitable surfaces for inducing MSCs to a chondrocyte or disc-like phenotype for tissue engineering of cartilage or IVDs, in which hypertrophy and osteogenesis are suppressed.

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Periodical:

Edited by:

Pietro VINCENZINI and Danilo DE ROSSI

Pages:

117-124

DOI:

10.4028/www.scientific.net/AST.57.117

Citation:

F. Mwale et al., "Novel Nitrogen Rich Polymers and Chitosan for Tissue Engineering of Intervertebral Discs", Advances in Science and Technology, Vol. 57, pp. 117-124, 2008

Online since:

September 2008

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