The preparation and charactersiation of novel lipid coated PLGA nanoparticles was investigated in the presented study. The size of the pure nanoparticles could be adjusted in dependence on the stabilizer content. The supported lipid bilayer surrounding the nanoparticles was formed by the liposome spreading technique. The Lipid- coated nanoparticles were characterised using Photon Correlation Spectroscopy (PCS) and atomic force microscopy (AFM) investigations. The bioadhesive properties was proofed in a cell culture model.