Electrochemical Detection of Bacteria Using Graphene Oxide Electrodeposited on Titanium Implants
Graphene oxide was electrodeposited on titanium (Ti-GO) and anodized titanium (ATi-GO) as label-free sensors for the detection of challenging living organisms, specifically Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The graphene modification contributed to two sets of oxidation-reduction peaks in cyclic voltammograms (CVs) of bacteria growth on the electrode surfaces (ATi-GO) that resulted in increasing direct electron transfer and stimulating excretion of mediating molecules for higher electron transfer between electrodes and bacteria. Additionally, similar wave patterns of CVs were found when E. coli or S. aureus were grown and electrocatalyzed on ATi-GO. The results suggest that bacteria on titanium implant surfaces could be easily detected by using mediatorless ATi-GO sensors electrochemically. These finding open another interesting method in using ATi-GO as in situ electrochemical sensors for label-free, close to real-time detection of bacteria infection in orthopedic implants.
S. Sirivisoot et al., "Electrochemical Detection of Bacteria Using Graphene Oxide Electrodeposited on Titanium Implants", Advances in Science and Technology, Vol. 96, pp. 45-53, 2014