Diffusion Kinetics of BSA Protein in Stimuli Responsive Hydrogels

Abstract:

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A two-dimensional diffusion mathematical model with moving boundary conditions was developed to evaluate the diffusion kinetics of bovine serum albumin (BSA) through the network of poly(N-isopropylacrylamide) hydrogel (poly(NIPAAm)). These thermosensitive hydrogels were experimentally tested for their response to BSA by exposing the hydrogel disc-shaped geometry to different temperatures and varied protein concentration. The BSA release, which is coupled with hydrogel shrinking when reaching the low critical solution temperature (LCST) of poly(NIPAAm), could be satisfactory described by the model. During the early course of hydrogel shrinking, the hydrogel outermost surface layer collapses to form a dense layer in comparison to the interior bulk matrix. Due to the hydrophobic interaction between polymer chains and polymer protein, the formed layer is thick and dense enough to restrict the outward permeation of entrapped BSA molecules from the hydrogel interior, which greatly slows down the release rate. A good agreement between experimental and calculated data was achieved.

Info:

Periodical:

Defect and Diffusion Forum (Volumes 297-301)

Edited by:

Andreas Öchsner, Graeme E. Murch, Ali Shokuhfar and João M.P.Q. Delgado

Pages:

664-669

DOI:

10.4028/www.scientific.net/DDF.297-301.664

Citation:

A.A. Naddaf et al., "Diffusion Kinetics of BSA Protein in Stimuli Responsive Hydrogels", Defect and Diffusion Forum, Vols. 297-301, pp. 664-669, 2010

Online since:

April 2010

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Price:

$35.00

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