Diffusion and Electrophoretic Transport of DNA Polymers in Microfluidic Channels Made of PDMS

Abstract:

Article Preview

DNA molecules can be transported through microchannels with help of electrophoresis and flow. Confinement of DNA molecules leads to elongation of their unconstrained equilibrium configuration when passing the microchannel. Application of electrical fields reduces the mobility and entails DNA trapping because of high gradients of the field due to a decrease in the channels’ magnitude. Microfluidic channels in polydimethylsiloxane (PDMS) were formed by soft replica molding technology combining micro- and nanofluidic features. The applicability of the hybrid micro- and nanofluidic PDMS structures for single molecule observation and manipulation was demonstrated by introducing single molecules of λ-DNA into the channels using optimized conditions for the applied potential and flow.

Info:

Periodical:

Defect and Diffusion Forum (Volumes 312-315)

Edited by:

Andreas Öchsner, Graeme E. Murch and João M.P.Q. Delgado

Pages:

1091-1096

DOI:

10.4028/www.scientific.net/DDF.312-315.1091

Citation:

R. Sczech et al., "Diffusion and Electrophoretic Transport of DNA Polymers in Microfluidic Channels Made of PDMS", Defect and Diffusion Forum, Vols. 312-315, pp. 1091-1096, 2011

Online since:

April 2011

Export:

Price:

$35.00

In order to see related information, you need to Login.

In order to see related information, you need to Login.