Simulation of In Vitro-Like Electrical Activities in Urinary Bladder Smooth Muscle Cells

Abstract:

Article Preview

Urinary bladder smooth muscle (UBSM) generates spontaneous electrical activities due to stochastic nature of purinergic neurotransmitter release from the parasympathetic nerve. The stochastic nature of the purinergic neurotransmitter release was represented by a simplified ‘point-conductance’ model to mimic in vitro-like electrical activities in UBSM cell. The point-conductance was represented by the independent synaptic conductance described by the stochastic random-walk processes and injected into a single-compartment model of mouse UBSM cell. This model successfully evoked irregular spontaneous depolarizations (SDs) and spontaneous action potential (sAP) as the properties of in vitro-like electrical activities in UBSM cells. The model mimics the T- and L-type Ca2+ ion channel blocker by setting their respective conductance to zero. We also found that the point-conductance model modulates the sAP properties by adding background activity.

Info:

Pages:

45-51

Citation:

C. Mahapatra and R. Manchanda, "Simulation of In Vitro-Like Electrical Activities in Urinary Bladder Smooth Muscle Cells", Journal of Biomimetics, Biomaterials and Biomedical Engineering, Vol. 33, pp. 45-51, 2017

Online since:

July 2017

Export:

Price:

$38.00

* - Corresponding Author

[1] Andersson, K-E. Purinergic signalling in the urinary bladder. , Autonomic Neuroscience 191 (2015): 78-81.

DOI: https://doi.org/10.1016/j.autneu.2015.04.012

[2] Brading, A. F. (2006). Spontaneous activity of lower Urinary tract smooth muscles: correlation between ion channels and tissue function. The Journal of physiology, 570(1), 13-22.

DOI: https://doi.org/10.1113/jphysiol.2005.097311

[3] Brading, A. F., & Brain, K. L. (2011). Ion channel modulators and urinary tract function. In Urinary Tract (pp.375-393). Springer Berlin Heidelberg.

DOI: https://doi.org/10.1007/978-3-642-16499-6_18

[4] Dave, V., Mahapatra, C., & Manchanda, R. (2015, August). A mathematical model of the calcium transient in urinary bladder smooth muscle cells. InEngineering in Medicine and Biology Society (EMBC), 2015 37th Annual International Conference of the IEEE (pp.5359-5362.

DOI: https://doi.org/10.1109/embc.2015.7319602

[5] Gabella, G. (1999). Structure of the intramural nerves of the rat bladder. Journal of neurocytology, 28(8), 615-637.

[6] Fellous, J. M., Rudolph, M., Destexhe, A., & Sejnowski, T. J. (2003). Synaptic background noise controls the input/output characteristics of single cells in an in vitro model of in vivo activity. Neuroscience, 122(3), 811-829.

DOI: https://doi.org/10.1016/j.neuroscience.2003.08.027

[7] Fry, C. H., et al. Measurement of intercellular electrical coupling in guinea-pig detrusor smooth muscle., The Journal of urology 161. 2 (1999): 660-664.

DOI: https://doi.org/10.1016/s0022-5347(01)61990-6

[8] Heppner, T. J., Bonev, A. D., & Nelson, M. T.: Elementary purinergic Ca2+ transients evoked by nerve stimulation in rat urinary bladder smooth muscle. The Journal of physiology, (2005) 564(1), 201-212.

DOI: https://doi.org/10.1113/jphysiol.2004.077826

[9] Hines, M. L., & Carnevale, N. T.: The NEURON simulation environment. Neural computation, (1997) 9(6), 1179-1209.

[10] Hodgkin, A. L., & Huxley, A. F.: A quantitative description of membrane current and its application to conduction and excitation in nerve. The Journal of physiology, (1952) 117(4), 500.

DOI: https://doi.org/10.1113/jphysiol.1952.sp004764

[11] Mahapatra, C., Brain, K. L., & Manchanda, R.: Electrophysiological Modeling of Electrical Activities in Detrusor Smooth Muscle Cells: Role of Purinergic Synaptic Input in Shaping Action Potential. In neurourology And Urodynamics  (2016).

[12] Mahapatra C., K. L. Brain, and Manchanda, R , Computational study of ATP gated Potassium ion channel in urinary bladder over activity., Inventive Computation Technologies (ICICT), International Conference on. Vol. 2. Pp. 1-4 IEEE, (2016).

DOI: https://doi.org/10.1109/inventive.2016.7824861

[13] Mahapatra, C., & Manchanda, R. (2017). MP42-01 QUANTITATIVE STUDY OF INWARD RECTIFYING ION CHANNEL IN DETRUSOR INSTABILITY. The Journal of Urology, 197(4), e544.

DOI: https://doi.org/10.1016/j.juro.2017.02.1290

[14] Mahapatra, C. (2017, April). Computational study of inward rectifying ion channel in urinary bladder over activity: student research abstract. InProceedings of the Symposium on Applied Computing (pp.31-32). ACM.

DOI: https://doi.org/10.1145/3019612.3019936

[15] Mahapatra, C., Brain, K., & Manchanda, R. (2015).

[16] Meng, En, John S. Young, and Alison F. Brading. Spontaneous activity of mouse detrusor smooth muscle and the effects of the urothelium., Neurourology and urodynamics 27. 1 (2008): 79-87.

DOI: https://doi.org/10.1002/nau.20456

[17] Young, J. S., Meng, E., Cunnane, T. C., & Brain, K. L.: Spontaneous purinergic neurotransmission in the mouse urinary bladder. The Journal of physiology, (2008) 586(23), 5743-5755.

DOI: https://doi.org/10.1113/jphysiol.2008.162040