Morphological Changes in the Upper Part of Digestive Tract in a Case of Experimental Duodenogastric Reflux

Romanenko, Y.G.; Komskyi, M.P.

doi:10.4028/www.scientific.net/JBBBE.35.77

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Morphological Changes in the Upper Part of Digestive Tract in a Case of Experimental Duodenogastric Reflux

Abstract:

Experimental reproduction of duodeno-gastric reflux was performed among Wistar line 28 rats, which were injected with 50 % solution of medical bile by intragastric way. Morphological study had been shown significant structural changes in the gingival tissues, mucous membrane of an esophagus, stomach and duodenum. In the epithelial layer of gingiva and esophagus was observed numerous infringements in a process of differentiation cells, in the stroma was shown phenomena of fibrosis in a papillary layer, microcirculation disorders. In the gastric mucosa had been found out multiple erosions, the glandular cells were increased. It was demonstrated vacuolization of the basal and thorn layers of the stomach epithelium. In the stroma defined phenomena of fibrosis and a dense lymphocyte infiltration with eosinophils. In the duodenum was determined desquamation of a glandular epithelium, superficial erosions of the villi, lympho–histio–plasmocytosis infiltration in a stroma, microvascular disorders. The given experiment demonstrates a role of functional disorders in the pathogenesis of combined pathology in the upper gastrointestinal tract.

Info:

Periodical:

Journal of Biomimetics, Biomaterials and Biomedical Engineering (Volume 35)

Main Theme:

Journal of Biomimetics, Biomaterials and Biomedical Engineering Vol. 35

Pages:

77-87

DOI:

https://doi.org/10.4028/www.scientific.net/JBBBE.35.77

Citation:

Y.G. Romanenko and M.P. Komskyi, "Morphological Changes in the Upper Part of Digestive Tract in a Case of Experimental Duodenogastric Reflux", Journal of Biomimetics, Biomaterials and Biomedical Engineering, Vol. 35, pp. 77-87, 2018

Online since:

January 2018

Authors:

Y.G. Romanenko *, M.P. Komskyi

Keywords:

Bile, Duodenum, Esophagus, Gingiva, Mucous Membrane, Reflux, Stomach

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Paper TitlePages

Encapsulation of Bifidobacterium bifidum in Improved Alginate Microcapsules to Prolonging Viability

Authors: Fan Zhang, Min Zhao, Wei Wang, Tie Feng Hu

Abstract: Bifidobacterium bifidum were encapsulated as fresh cultures in water insoluble food grade microcapsules by emulsification–internal gelation technique, using gelatin, pectin, alginate and chitose as immobilization material. The optical photomicroscope photograph and laser scanning confocal microscope photograph shows that the microcapsules obtained by this method has narrow size distribution (10-20 μm). Survival of Bifidobacterium bifidum in microcapsules was 109cfu•g^-1 , and the embedding ratio was 72.32%. The survival was remaining more than 108cfu•g^-1 after exposure to gastric acid and bile acid, which implies that the microcapsules expressed superior acid resistance. These microcaosules were disaggregation subtotal after 15 min in simulated intestinal juices, and the release rate was 96%. The survival was 108 cfu•g^-1 at room temperature after 1 year by classic accelerated test. The results indicate that encapsulation of Bifidobacterium bifidum in alginate microcapsules is a good approach to prolonging viability.

1481

Screening of Bile Salt Hydrolase-Active Lactic Acid Bacteria for Potential Cholesterol-Lowering Probiotic Use

Authors: Chun Feng Guo, Lan Wei Zhang, Jing Yan Li, Ying Chun Zhang, Chao Hui Xue, Hua Xi Yi, Ming Du, Xue Han

Abstract: .Cholesterol-lowering effect of lactic acid bacteria (LAB) with bile salt hydrolase activity is well known. In this study, 150 LAB were screened for bile salt deconjugation ability and probiotic characters. Fourteen isolates with higher bile salt deconjugation ability were initially screened out using deconjugation rate above 50% as standard. These isolates were further screened for adhesion to HT-29 cells, bile tolerance and acid resistance. Four isolates, namely Lactobacillus casei F0822, Lactobacillus casei F0422, Enterococcus faecium F0511 and Enterococcus faecium IN7.12, was finally screened out. The 4 isolates may be able to reduce serum cholesterol levels in human and thus have a potential to apply in the biomedicine field.

139

Screening for Antiproliferative Effect of Lactobacillus Strains Against Colon Cancer HT-29 Cells

Authors: Shu Mei Wang, Lan Wei Zhang, Wei Gu, Chao Hui Xue, Ying Chun Zhang, Li Li Zhang, Xue Luo, Hong Bo Li

Abstract: One hundred and forty lactobacillus strains which were obtained from infant faeces and traditional fermented foods in western China were examined for possible use as probiotics. The live whole cells from lactobacilli were tested for antiproliferative effects, adherent capability, acid and bile tolerance on colon cancer HT-29 cells. The results showed that eleven strains inhibited growth of HT-29 cells. Among the eleven strains, ten strains were further screened out through adhering activity assay. All the ten strains could grow in acidified broth at pH 1.5 and in 0.3% (w/v) bile salt. Nevertheless, G5, X11, X12, M5, M23, K11 and K14 showed the most profound inhibitory activity against HT-29 cells. While Q12, SB27, X2 and M7 were poorly presented. 16S rRNA gene sequences indicated that X12, M5, M7 and M23 were Lactobacillus paracasei, G5, Q12, SB27, X2, X11 and K14 were Lactobacillus casei, K11 was Lactobacillus rhamnosus. Together, these results suggest G5, X11, X12, M5, M23, K11 and K14 to be good probiotic candidates and potential application in functional foods and health-associated products.

1039

In Vitro Comparison of Probiotic Properties of Lactobacillus fermentum SK54 Isolated from New Born Baby with Lactobacillus rhamnosus GG ATCC 53103

Authors: Kanyanat Kaewiad, Sanae Kaewnopparat, Nattha Kaewnopparat

Abstract: A lactobacillus strain isolated from the feces of a healthy baby was identified as Lactobacillus fermentum SK54. The probiotic properties of this strain including antimicrobial activity, survival in acid, bile, simulated gastric fluid [SGF] and simulated intestinal fluid [SIF], antibiotic susceptibility and adherence to Caco-2 cells were examined and compared them with a well known commercial probiotic strain Lactobacillus rhamnosus GG ATCC 53103 isolated from human intestine. L. fermentum SK54 and L. rhamnosus GG showed inhibitory properties toward gastrointestinal pathogens i.e., Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella Typhimurium, and Shigella sonnei by cylinder-plate assay. The inhibitory substances from L. fermentum SK54 composed of acid substances naturally produced by lactobacilli and high molecular weight proteinaceous compound [MW > 3.5 kDa] which sensitive to proteolytic enzymes, pH stability [pH 2.0-5.0] and heat stability [60, 80, 100°C for 10 min and 121°C for15 min]. L. rhamnosus GG produced only acid inhibitory substances. Both strains survived well at low pH [pH 2.0-4.0], bile salts [0.1-0.3% w/v], SGF and SIF. They also had the same 14 tested antibiotic sensitivity pattern and expressed high adherence to Caco-2 cells. This study provides a rationale to support L. fermentum SK54 as a potential probiotic to prevent or act as a therapeutic agent against pathogenic strains.

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