Marrow Mesenchymal Stem Cells Cultured on Alumina Ceramics (From Basic Science to Clinical Applications)

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Periodical:

Key Engineering Materials (Volumes 240-242)

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Edited by:

Besim Ben-Nissan, Doron Sher, William Walsh

Pages:

651-654

DOI:

10.4028/www.scientific.net/KEM.240-242.651

Citation:

H. Ohgushi et al., "Marrow Mesenchymal Stem Cells Cultured on Alumina Ceramics (From Basic Science to Clinical Applications)", Key Engineering Materials, Vols. 240-242, pp. 651-654, 2003

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May 2003

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[14] Y. Takakura, Y. Tanaka, T. Takaoka, M. Okumura, H. Ohgushi, S. Tamai, Bioceramics 5: 459-464, 1993(Kobunshi Kankokai, Inc.: Editors, T. Yamamuro, T. Kokubo and T. Nakamura) Acknowledgments We thank Kyocera Co. Kyoto, Japan for providing the alumina ceramics. This work was supported by Three-Dimensional Tissue Module Project, METI (A Millenium Project). Fig. 3 Human MSCs culture on artificial ankle prosthesis made by alumina ceramics. The MSCs obtained from 65 years patient were loaded on the ceramic prosthesis and subcultured in the presence of vitamin C, glycerophosphate and dexamethason (left figure). The subcultured cells on the prosthessi was then subjected for ALP stain (right figure). The red color indicates the ALP positive areas. Note that about 80% surface area is covered with ALP positive stain.

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