Optimization of Vibrio harveyi Luminometry Assay for Detecting Quorum Sensing Inhibitors
The luminometry assay using the wild-type Vibrio harveyi BB120 was evaluated as a possible detection method for quorum sensing inhibitors. The effects of the concentration of the quorum sensing signal molecule (AHL) as well as the cell density of the reporter strain and the different AHL analogues on luminescence expressed as relative light units (RLU) were examined. Inhibition of V. harveyi luminescence was observed in a dose dependent manner for all five AHL analogues. The RLU values exhibited linearity within the range of 2.9 x 102 ~ 3.2 x 105. Detection up to 102nM was possible for dodecanoyl-homoserine lactone and AHLs with alkyl chain lengths of C-8~C-14 were more active than the shorter chain-lengthed hexanoyl-homoserine lactones. Lipophilicity of the AHL seems to affect the sensitivity of the assay.
Kwang Hwa Chung, Yong Hyeon Shin, Sue-Nie Park, Hyun Sook Cho, Soon-Ae Yoo, Byung Joo Min, Hyo-Suk Lim and Kyung Hwa Yoo
Y. H. Kim et al., "Optimization of Vibrio harveyi Luminometry Assay for Detecting Quorum Sensing Inhibitors", Key Engineering Materials, Vols. 277-279, pp. 19-22, 2005