A Study of the Biosorption Characteristics of Co2+ in Wastewater Using Pseudomonas Aeruginosa


Article Preview

Pseudomonas aeruginosa biomass was used to investigate the biosorption properties of heavy metals in wastewater. The biosorption isotherm of Co2+ was best described by the Langmuir model when washed cells were employed, and results obtained utilizing heat-treated P. aeruginosa were also adequately represented by a Langmuir sorption isotherm. In contrast, the sorption isotherm involving unwashed P. aeruginosa showed a different isotherm profile and did not attain equilibrium in the range of metal concentrations investigated; the amount of Co2+ uptake increased with increasing initial metal concentration but never reached adsorption equilibrium, most likely due to bacterial production of extracellular polymeric substances (EPS). The biosorption results utilizing unwashed P. aeruginosa were best described by a Freundlich isotherm. The level of metal adsorption in low pH was significantly small due to competition between the cation and H+ ions for binding sites distributed on cell surfaces, while the increase in pH favored metal sorption because of the elevated quantities of negatively charged surface functional groups. The sorption of Co2+ was strongly influenced by the presence of competing cations in the solution. Trivalent Cr3+ added to the solution was preferentially adsorbed onto the cells relative to Co2+ and Ni2+. The results may be attributed to the higher adsorption affinity of Cr3+ in comparison to either Co2+ or Ni2+. The affinity order (Cr3+ > Co2+ » Ni2+) was maintained over a pH range up to 5.3 in a mixture solution.



Key Engineering Materials (Volumes 277-279)

Edited by:

Kwang Hwa Chung, Yong Hyeon Shin, Sue-Nie Park, Hyun Sook Cho, Soon-Ae Yoo, Byung Joo Min, Hyo-Suk Lim and Kyung Hwa Yoo




S. Y. Kang et al., "A Study of the Biosorption Characteristics of Co2+ in Wastewater Using Pseudomonas Aeruginosa ", Key Engineering Materials, Vols. 277-279, pp. 418-423, 2005

Online since:

January 2005




[1] D.C. Adriano: Trace Elements in Terrestrial Environments (Springer-Verlag, New York 2001).

[2] M. Lehmann, A.I. Zouboulis and K.A. Matis: Chemosphere Vol. 39 (1999), p.881.

[3] B. Volesky: Biosorption of Heavy Metals (CSC Press, Boston 1989).

[4] G.M. Gadd: J. Chem. Technol. Biotechnol. Vol. 55 (1992) p.302.

[5] T.J. Beveridge: Int. Rev. Cytology. Vol. 72 (1981), p.229.

[6] H.L. Ehrlich: Appl. Microbiol. Biotechnol. Vol. 48 (1997), p.687.

[7] W. Stumm and J.J. Morgan: Aquatic Chemistry: Chemical Equilibria and Rates in Natural Waters (Wiley Interscience, New York 1996).

[8] S. Langley and T.J. Beveridge: Can. J. Microbiol. Vol. 45 (1999), p.616.

[9] G.W. Strandberg, S.E. Shumate, and J.R. Parrott: Appl. Environ. Microbiol. Vol. 41 (1981), p.237.

[10] I.H. Çeribasi and Ű. Yetis: Water SA Vol. 27 (2001), p.15.

[11] J.B. Fein, C.J. Dauchney, N. Yee, and T.A. Davis: Geochimica et Cosmochimica Acta Vol. 61 (1997), p.3319.

[12] N. Kuyucak and B. Volesky: Biotechnol. Bioeng. Vol. 33 (1989), p.809.