Antimicrobiologic Property Hydrated Amorphous Calcium Phosphates Containing Silver


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The purpose of this study was to determine the microbiologic effect silver (Ag) when incorporated in amorphous calcium phosphate (ACP) prepared from solutions containing calcium, phosphate and pyrophosphate ions. The preparations were obtained from solutions with phosphate/pyrophosphate ratio, P/P2 = 7/3, and characterized as ACP using X-ray diffraction, infrared spectroscopy, nuclear magnetic resonance and differential thermal analysis. The microbes tested were cultures of E. Coli, St. aureus, Ps aerogunosa, C. diphteroides and C. albicans in media alone (control), with ACP without Ag and with ACP containing different Ag concentrations. Results demonstrated the following: (1) Ag in ACP inhibited the growth of microorganisms in all five cultures, with ACP containing 3-6% Ag giving the greatest antimicrobial effect; (2) sensitivity to the antimicrobial action of Ag differed among the bacteria.



Key Engineering Materials (Volumes 284-286)

Main Theme:

Edited by:

Panjian Li, Kai Zhang and Clifford W. Colwell, Jr.




O.J. Aimanova et al., "Antimicrobiologic Property Hydrated Amorphous Calcium Phosphates Containing Silver", Key Engineering Materials, Vols. 284-286, pp. 439-444, 2005

Online since:

April 2005




[1] A Pommer, M.P. Hahn, G. Muhr: Am Acad Orthoped Surgeons 67th Annual Meeting, March 15- 19 (2000), abstract #72.

[2] A.M. Mulligan, M. Wilson, J.C. Knowles. J. Biomed. Mater. Res. (2003) 67A: 401-412.

[3] T. Gilchrist, D.M. Healy, C. Drake. Biomaterials (1991) 12: 76-78.

[4] U. Klueh, V. Wagner, S. Kelly, A. Johnson, J.D. Bryers. J. Biomed. Mater. Res. (Appl. Biomat. ) (2000) 53: 621-631.

[5] R.Z. LeGeros, W.P. Shirra, M.A. Miravite, J.P. LeGeros. CNRS No. 230. Physico-chimie et Crystallographie des Apatites d'Interet Biologique. Paris (1973), pp.105-115.

[6] R.Z. LeGeros, X-F. Chang. J. Dent. Res. (1989) 68: 215.

[7] R.Z. LeGeros Clin. Mat. (1993) 14: 65-88.

[8] R.Z. LeGeros. Calcium Phosphates in Oral Biology and Medicine. (1991). Basel: Karger.

[9] R.Z. LeGeros, C.B. Bleiwas, M. Retino, R. Rohanizadeh, J.P. LeGeros. Am. J. Dent. (1999) 12: 65-70.

[10] E.N. Silina, L.V. Levchenko, G.A. Temiralieva, T.N. unitsa, J. Griggs, V.A. Sinyayev. Reports of the National Academy of Sciences of Kazakhstan (2003) 2: 55-60 (in Russian).

[11] R.S. Musagalieva, A.A. Abdirasilova, L.V. Levchenko, G.A. Temiralieva, TN. Kunitsa, J. Griggs, V.A. Sinyayev. Reports of the National Academy of Sciences of Kazakhstan (2004) 2 (in Russian).

[12] M.O. Birger. Reference Book on Microbiological and Virological Techniques of Investigation. (1982) Moscow Medical Publishing, pp.40-96 (in Russian).

[13] R.A. Harper, A.S. Posner. Mater Res Bull (1970) 5: 129-136.