In Vitro Analysis of Protein Adhesion to Phase Pure Hydroxyapatite and Silicon Substituted Hydroxyapatite


Article Preview

The adhesion of bovine collagen type I, bovine serum albumin, bovine IgG, 1 % and 10 % (v/v) human serum to hydroxyapatite (HA), silicon-substituted hydroxyapatite (Si-HA) and tissue culture plastic were studied. The materials were incubated at 37 °C for 30 minutes, after which the protein solution was removed and analyzed. The adsorbed protein was evaluated by electrophoresis and immunoassay after extraction from the materials. The degree of adhesion was higher for collagen, followed by IgG and albumin on all materials. However there was no difference in the amount of collagen adsorbed onto the surface of each material and this was also the finding with albumin and IgG. These results suggest that the increased bioactivity seen with Si-HA is not due to the degree of protein adhesion, but may possibly be due to changes in the conformation of the bound proteins.



Key Engineering Materials (Volumes 284-286)

Main Theme:

Edited by:

Panjian Li, Kai Zhang and Clifford W. Colwell, Jr.




C.M. Botelho et al., "In Vitro Analysis of Protein Adhesion to Phase Pure Hydroxyapatite and Silicon Substituted Hydroxyapatite ", Key Engineering Materials, Vols. 284-286, pp. 461-464, 2005

Online since:

April 2005




[1] Patel et al, J. of Mat. Sci.: Mat. in Med., Vol. 13 (2002) p.1199.

[2] I.R. Gibson et al, in Bioceramics, Vol. 12, (1999), p.191.

[3] C.M. Botelho et al, in Key Engineering Materials, Vol. 254-256, (2003), pp.841-844.

[4] Porter et al, in Biomaterials, Vol. 5, (2004), pp.3303-3314.

[5] Takami et al, in J. Biomed Mater. Res., Vol. 40, (1998), pp.24-30.

[6] Kandori et al, in J. Colloid and Interface Science, Vol 273, (2004), pp.406-413.

[7] Vermeer et al, Biochimica et Biophysica Acta Vol 1526 (2001), pp.61-69.

[8] Kikuchi et al, Biomaterials, Vol 22, (2001), pp.1705-1711.

[9] L.J. Jha, et al, Worldwide patent, PCT/GB97/02325 and US Patent Serial Nº 09/147773, (1999).

[10] I.R. Gibson et al, J. Biomed. Mat. Res., Vol. 44, (1999), p.422.

[11] C. M. Botelho et al, J. Mater. Sci: Mater. Med, Vol. 13 (2002), pp.1123-1127.

[12] Muller et al, Macromol. Rapid Commun. Vol. 20, (1999), pp.607-611.

[13] Veerman et al, in Biomaterials, Vol. 8, (1987), pp.442-448.

[14] Haynes et al, in Colloids and Surfaces B: Biointerfaces, Vol. 2 , (1994) 517-566.

[15] D. M. Reffit et al, Bone, Vol. 32, (2003).