A Bio-Photonics System for Rapid In Vitro Testing of Cells and Ceramics
We present a new bio-photonic method based on Raman spectroscopy able to characterize living cells in in-vitro cultures. The main advantages of this technology are: no labels or other contrast enhancers are required; provides real-time analysis; cells can be maintained in physiological conditions during the measurements; no cell-damage is induced during the measurements; it is rich in information about the biochemical composition of the cell. The results show that this spectroscopic method can be used to study the most important cellular functions involved in cell-biomaterial interactions, such as cell death, differentiation, de-differentiation and mineralization. The method offers the potential for studying cell-bioceramics interaction and reduce the need of animal testing until the final steps of proving efficacy prior to clinical trials.
Panjian Li, Kai Zhang and Clifford W. Colwell, Jr.
L. L. Hench and I. Notingher, "A Bio-Photonics System for Rapid In Vitro Testing of Cells and Ceramics", Key Engineering Materials, Vols. 284-286, pp. 531-536, 2005