A Bio-Photonics System for Rapid In Vitro Testing of Cells and Ceramics


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We present a new bio-photonic method based on Raman spectroscopy able to characterize living cells in in-vitro cultures. The main advantages of this technology are: no labels or other contrast enhancers are required; provides real-time analysis; cells can be maintained in physiological conditions during the measurements; no cell-damage is induced during the measurements; it is rich in information about the biochemical composition of the cell. The results show that this spectroscopic method can be used to study the most important cellular functions involved in cell-biomaterial interactions, such as cell death, differentiation, de-differentiation and mineralization. The method offers the potential for studying cell-bioceramics interaction and reduce the need of animal testing until the final steps of proving efficacy prior to clinical trials.



Key Engineering Materials (Volumes 284-286)

Main Theme:

Edited by:

Panjian Li, Kai Zhang and Clifford W. Colwell, Jr.




L. L. Hench and I. Notingher, "A Bio-Photonics System for Rapid In Vitro Testing of Cells and Ceramics", Key Engineering Materials, Vols. 284-286, pp. 531-536, 2005

Online since:

April 2005




[1] L.L. Hench, J. Wilson eds: Clinical Performance of Skeletal Prosthesis, Chapman & Hall, London (1996).

[2] L.L. Hench: Science 208 (1980), p.826.

[3] L.L. Hench, J.M. Polak: Science 295 (2002), p.1014.

[4] I. Notingher, S. Verrier, H. Romanska, A. E. Bishop, J. M. Polak, L. L. Hench: Spectrosc. -Int.J. 16 (2002), p.43.

[5] I. Notingher, S. Verrier, S. Haque, J.M. Polak, L.L. Hench: Biopolymers 72 (2003), p.230.

[6] I. Notingher, J. E. Gough, L. L. Hench: Key Engineering Materials 254-256 (2003), p.769.

[7] R. Wolthuis, T.C. Bakker Schut, P.J. Caspers, H.P.J. Buschman, T.J. Romer, H.A. Bruining, G.J. Puppels: In: Fluorescent and luminescent probes for biological activity, Mason WT ed. Academic Press, 1999, London, p.433.

DOI: https://doi.org/10.1016/b978-012447836-7/50034-8

[8] K.E. Shafer-Peltier, A.S. Haka, J.T. Motz, M. Fitzmaurice, R.R. Dasari, M.S. Feld: J. Cell. Biochem. 39 (2002) p.125.

[9] I. Notingher, I. Bisson, A. E. Bishop, W. L. Randle, J. M. P. Polak, L. L. Hench: Anal. Chem. 76 (2004), p.3185.

[10] I. Notingher, G. Jell, U. Lohbauer, V. Salih, L. L. Hench: J. Cell. Biochem. (2004), in press (online).

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