The Effect of Cell Behavior on Apatites with Different Fluorine Contents

Abstract:

Article Preview

Fluoridated hydroxyapatite (FHA) discs with various fluorine contents have been used to study the effect of fluorine content on early-stage cell behavior. FHA powders with fluorine content in the range 0-0.577 (mol F /mol apatite) were pressed into discs and sintered at 1200°C for 1 hour. SAOs-2 rat osteosarcoma cells were cultured on each FHA disc and tissue culture polystyrene (control) with the same seeding density for 4 hours. The cell count was conducted using Alamarblue, and the morphology of cell attachment was observed using environmental scanning electron microscopy. It was apparent that the fluorine content in FHA had significant impact on the early cell behavior.

Info:

Periodical:

Key Engineering Materials (Volumes 284-286)

Main Theme:

Edited by:

Panjian Li, Kai Zhang and Clifford W. Colwell, Jr.

Pages:

577-580

Citation:

H. B. Qu et al., "The Effect of Cell Behavior on Apatites with Different Fluorine Contents", Key Engineering Materials, Vols. 284-286, pp. 577-580, 2005

Online since:

April 2005

Export:

Price:

$38.00

[1] L.L. Hench, J. Wilson: An Introduction to Bioceramics, World Scientific, (1993).

[2] M. Okazaki, Y. Miake, H. Tohda, T. Yanagisawa, T. Matsumoto, J. Takahashi, Biomaterials 20(1999)1421.

DOI: https://doi.org/10.1016/s0142-9612(99)00049-6

[3] M. Sundfeldt, M. Widmark, A. Wennerberg, J. Kärrholm, C. B. Johansson, L.V. Carlsson: J Mater Sci Mater Med. 13(2002)1037.

[4] K. Lau, C. Goodwin, M. Arias, S. Mohan, D. Baylink: Bone 30 (2002) 705-711.

[5] K. H. W. Lau, K. Åkesson, C. R. Libanati, D. J. Baylink, In: J. F. Whitfield and P. Morley Eds: Anabolic Treatments for Osteoporosis. Boca Raton, CRC Press, (1998).

[6] H. Qu, M. Wei: J Mater Sci Mater Med, submitted.

[7] T. Gao, H. T. Aro, H. Ylänen, E. Vuorio: Biomaterials 22(2001)1475-1483.

[8] K. Eggen, G. Rolla: Scandinavian Journal of Dental Research, 91(1983) 347-350.

[9] O. Nakade, H. Koyama, J. Arai, H. Ariji, J. Takada, T. Kaku: Achives of Oral Biology 44(1999)89-92.

[10] K. C. Dee, D. A. Puleo, R. Bizios: An Introduction to Tissue-Biomaterial Interactions, John Wiley & Sons, (2002).

DOI: https://doi.org/10.1002/0471270598

[11] T. M. Lee, E. Chang, C. Y. Yang: Biomaterials 25(2004)23-32.

[12] K. A. Gross, K. A. Bhadang: Biomaterials 25(2004)1395-1405.