Behavior of Osteoblasts Cultured on Collagen Coated Bioactive Glass
In the current work, we investigated cellular viability, proliferation, and metabolic activity of rat primary culture osteoblasts in contact with a sample of collagen type I (C) and of this same collagen chemically treated (CTP). The chemical process used here consisted in recover the collagen surface with silica glass obtained from a sol-gel process. The cell viability, the cell death, the alkaline phosphate production and collagen secretion, after 72 hours of incubating the samples with osteoblasts, were measured by MTT assay, propidium iodide, NBT-BCIP assay and SIRCOL method, respectively. The viability and proliferation of osteoblasts had a significant decrease in the presence of samples when compared to control. The alkaline phosphatase production by the cells had a significant increase in the presence of CTP and collagen secretion by the cells was practically the same when compared to control.
Panjian Li, Kai Zhang and Clifford W. Colwell, Jr.
A.L. Andrade et al., "Behavior of Osteoblasts Cultured on Collagen Coated Bioactive Glass", Key Engineering Materials, Vols. 284-286, pp. 675-678, 2005