Carboxymethylchitosan/Calcium Phosphate Hybrid Materials Prepared by an Innovative Auto-Catalytic Co-Precipitation Method
In this study, it is shown that it is possible to prepare carboxymethyl-chitosan/Ca-P hybrids using an innovative “auto-catalytic” co-precipitation method, namely by using an acid and an oxidant bath. The X-ray diffraction (XRD) patterns evidenced the formation of crystalline calcium-phosphate precipitates when using an acid bath, while amorphous ones were obtained for those produced in the oxidant bath. The Fourier Transform Infrared spectroscopy (FTIR) and Scanning Electron Microscopy (SEM/EDS) studies revealed that the extent of the polymer precipitation and formation of calcium-phosphates is directly dependent on the pH and composition of the baths. Furthermore, by conducting bioactivity tests in a simulated body fluid (SBF) followed by the SEM/EDS analysis it was possible to detect the formation of an apatite layer with a cauliflower-like morphology on the surface of hybrids prepared by the acid bath, after 7 days of immersion. These results are quite promising because they can allow for the production of bioactive and biodegradable 3D porous scaffolds to be used in bone tissue engineering applications.
Panjian Li, Kai Zhang and Clifford W. Colwell, Jr.
S.A. Costa et al., "Carboxymethylchitosan/Calcium Phosphate Hybrid Materials Prepared by an Innovative Auto-Catalytic Co-Precipitation Method ", Key Engineering Materials, Vols. 284-286, pp. 701-704, 2005