Construction of Biodegradable Multilayer Films via Layer-by-Layer Self-Assembly as Gene Delivery System
Films composed of alternating layers of protamine and DNA were constructed using the layer-by-layer method on quartz and subsequently studied the enzymic degradation in vitro. UV-visible spectrometry measurement indicated the uniform assembly of Protamine/DNA multilayer films. UV-visible spectrometry and fluorescence spectrometry results revealed that the Protamine/DNA multilayer films were in vitro enzymic biodegradable. The novel biodegradable multilayer of Protamine/DNA may have great potential for gene therapy applications in tissue engineering, medical implant etc.
Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata
K. F. Ren et al., "Construction of Biodegradable Multilayer Films via Layer-by-Layer Self-Assembly as Gene Delivery System", Key Engineering Materials, Vols. 288-289, pp. 101-104, 2005