Construction of Biodegradable Multilayer Films via Layer-by-Layer Self-Assembly as Gene Delivery System


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Films composed of alternating layers of protamine and DNA were constructed using the layer-by-layer method on quartz and subsequently studied the enzymic degradation in vitro. UV-visible spectrometry measurement indicated the uniform assembly of Protamine/DNA multilayer films. UV-visible spectrometry and fluorescence spectrometry results revealed that the Protamine/DNA multilayer films were in vitro enzymic biodegradable. The novel biodegradable multilayer of Protamine/DNA may have great potential for gene therapy applications in tissue engineering, medical implant etc.



Key Engineering Materials (Volumes 288-289)

Edited by:

Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata




K. F. Ren et al., "Construction of Biodegradable Multilayer Films via Layer-by-Layer Self-Assembly as Gene Delivery System", Key Engineering Materials, Vols. 288-289, pp. 101-104, 2005

Online since:

June 2005




[1] Decher, G.: Science. Vol. 277 (1997), pp.1232-1237.

[2] Hammond, P. T.: Curr. Opin. Colloid Interface Sci. Vol. 3 (1998), pp.32-39.

[3] Sukhishvili, S. A.; Granick, S.: Macromolecules. Vol. 35 (2002), pp.301-310.

[4] Schüler, C., Caruso, F.: Biomacromolecules. Vol. 2 (2001), pp.921-926.

[5] Eduardo Vázquez., David M. Dewitt., Paula T. Hammond., David M. Lynn.: J. Am. Chem. Soc. Vol. 124 (2002), pp.13992-13993.

[6] Serizawa T., Yamaguchi M., Akashi M.: Macromolecules. Vol. 35 (2002), pp.8656-8658.

[7] Xiangyang Shi., Raymond J. Sanedrin., Feimeng Zhou.: J. Phys. Chem. B Vol. 106 (2002), pp.1173-1180.

[8] LePecq, J. B., Paoletti, C.: Anal. Biochem. Vol. 17 (1966), pp.100-107.

[9] Dutton, M. D., Varhol, R. J., Dixon, D. G.: Anal. Bio-chem. Vol. 230 (1995), pp.353-355.