Gene Expression of C2C12 Cells on Calcium Phosphate Ceramic In Vitro
The osteoinduction of Calcium Phosphate (CaP) had been proved and generally been investigated by in vivo implantation. However, the mechanism of the osteoinductivity was not clear and it was difficult to judge the osteoinductivity in vitro. In this study, Mouse C2C12 cell line, a kind of myoblast precursor cell, was employed to co-culture with CaP. The induction of cell differentiation by materials was tested by MTT method, fluorescence observation, especially the mRNA expression of Osteocalcin, Type I collagen and Fibronectin by RT-PCR. It was founded that C2C12 cells could be induced to expression osteocalcin when growth on the surface of the HA/TCP ceramics. At the same time, the ceramics with different composition and sintering temperature seemed to induce difference expression level of the related genes. The results proved that phase composition was one of the most important factors in the regulation of bone-related genes. This study provided a potential model to evaluate the osteoinductivity of CaP ceramics in vitro.
Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata
Y. F. Tan et al., "Gene Expression of C2C12 Cells on Calcium Phosphate Ceramic In Vitro", Key Engineering Materials, Vols. 288-289, pp. 265-268, 2005