Preparation of Porous Multi-Channeled Chitosan Conduits for Nerve Tissue Engineering

Abstract:

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A new method to fabricate porous chitosan nerve conduits with multi-channels was described. A uniquely designed mold was composed of 7-50 stainless steel needles and a set of plastic pedestals. Porous or imperforate chitosan tubes with 2-5mm inner diameter and 0.2-1.0 mm wall thickness were made firstly. The chitosan tubes were injected with 3% chitosan gel. The stainless steel needles longitudinally perforated through the chitosan tubes filled with chitosan gel, and the plastic pedestals were used to fix the needles. Lyophilization was used to finish fabrication. The diameter of channels was 0.2-0.4mm. Swelling property and biodegradability of Multi-channeled chitosan conduits were investigated. Wright’s staining and scanning electron microscope (SEM) were used to observe spread and proliferation of Neuroblastoma cells (N2A, mouse) on the conduits. It is promising that the porous chitosan nerve conduits with multi-channels are used as nerve tissue engineering scaffolds in repair of peripheral nerve and spinal cord injuries.

Info:

Periodical:

Key Engineering Materials (Volumes 288-289)

Edited by:

Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata

Pages:

27-30

Citation:

Q. Ao et al., "Preparation of Porous Multi-Channeled Chitosan Conduits for Nerve Tissue Engineering", Key Engineering Materials, Vols. 288-289, pp. 27-30, 2005

Online since:

June 2005

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Price:

$38.00

[1] Den Dunnen WF, Meek MF, Grijpma DW, Robinson PH, Schakenraad JM: J Biomed Mater Res 2000; 51: 575 -585.

[2] Kim SE, Park JH, Cho YW, Chung H, Jeong SY, Lee EB, Kwon IC: J Control Release 2003; 91(3): 365-374.

[3] Teng YD, Lavik EB, Qu X, Park KI, Ourednik J, Zurakowski D, Langer R, Snyder EY: PNAS 2002; 99(5): 3024-3029.

[4] Gong HP, Zhong YH, Li JC, Gong YD, Zhao NM, Zhang XF: J Biomed Mater Res 2000; 52: 285-295.

[5] Li JC, Zhong YH, Gong HP, Yang M, Gong YD, Zhao NM, Zhang XF: The Fifth IUMRS International Conference on Advanced Materials, June 13-18, 1999, Beijing, China.

[6] Madihally SV, Matthew HWT: Biomaterials 1999; 20: 1133-1142.

[7] VandeVord PJ, Matthew HW, DeSilva SP, Mayton L, Wu B, Wooley PH: J Biomed Mater Res 2002; 59(3): 585-590.

[8] Zhang Y, Zhang MQ: J Biomed Mater Res, 2001; 55: 304-312.

[9] Schoof H, Apel J, Heschel I, Gunter R: J Biomed Mater Res 2001; 58: 352-357.

[10] Nam YS, Park TG: Biomaterials 1999; 20: 1783-1790.

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