Attachment and Proliferation of Human Dermal Fibroblasts onto ECM-Immobilized PLGA Films


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In this study, human dermal fibroblast behaviors onto non-porous PLGA (75:25) films immobilized with 1, 10 and 100 µg/ml collagen (CN) or fibronectin (FN) were investigated according to different cell-seeding densities (1,000, 10,000 and 100,000 cells/ml). Cell attachment and proliferation were assessed using water soluble tetrazolium salt. The results indicated that 1 µg/ml of FN-immobilized PLGA film demonstrated significantly (p < 0.05) superior cellular attachment to the intact PLGA film after 4 hr of incubation. Moreover, the number of attached cells was shown to be directly proportional to that of initially seeded cells. After 48 hr, the cells showed significantly (p < 0.05) higher proliferation onto 1 or 10 µg/ml of FN-immobilized PLGA films than onto other PLGA films, regardless of the initial cell-seeding density. In terms of CN-immobilization, cell proliferation was appreciably increased but it was relatively lower than FN-immobilization. These results suggested that ECM-immobilization can enhance the cell affinity of hydrophobic scaffolds and be used to potential applications for tissue engineering by supporting cell growth.



Key Engineering Materials (Volumes 288-289)

Edited by:

Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata




H. J. Son et al., "Attachment and Proliferation of Human Dermal Fibroblasts onto ECM-Immobilized PLGA Films", Key Engineering Materials, Vols. 288-289, pp. 291-294, 2005

Online since:

June 2005




[1] R. Langer and J.P. Vacanti: Science Vol. 260 (1993), p.920.

[2] J. Yang, G. Shi, J. Bei, et al: J. Biomed. Mater. Res. Vol. 62 (2002), p.438.

[3] S.B. Lee, H.W. Jeon, Y.M. Lee et al: Biomaterials Vol. 24 (2003), p.2503.

[4] K.W. Ng, H.L. Khor and D.W. Hutmacher: Biomaterials Vol. 25 (2004), p.2807.

[5] M.D. Evans and J.G. Steele: J. Biomed. Mater. Res. Vol. 40 (1998), p.621.

[6] A.G. Mikos, G. Sarakinos, S.M. Leite, et al: Biomaterials Vol. 14 (1993), p.323.

[7] J. -C. Park, B.J. Park, H. Suh, B.Y. Park and D.K. Rah: Yonsei Med. J. Vol. 42 (2001), p.587.

[8] Y. Kanemura, H. Mori, S. Kobayashi, et al: J. Neurosci. Res. Vol. 69 (2002), p.869.

[9] A.G. A Coombes, E. Verderio, B. Shaw, et al: Biomaterials Vol. 23 (2002), p.2113.

[10] K. Cai, K. Yao, Y. Cui, et al: Biomaterials Vol. 23 (2002), p.1603.

[11] I. -K. Kang, S. -H. Choi, D. -S. Shin and S.C. Yoon: Int. J. Biol. Macromol. Vol. 28 (2001), p.205.

[12] K. Inouye, M. Kurokawa, S. Nishikawa and M. Tsukada: J. Biochem. Biophys. Methods Vol. 37 (1998), p.159.