Effect of Fibrin/Collagen Matrix on Osteogenic Differentiation of Bone Marrow Stromal Cells


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Fibrin is a natural polymer with excellent biocompatibility and widely used as a cell delivery vehicle in tissue engineering. However, fibrin of low concentration is not able to promote cell growth and differentiation within a desired time because of contraction and biodegradation of cell-seeded matrices. In this study we investigated effects of combining fibrin with collagen on growth and osteogenic differentiation of bone marrow stromal cells (BMSCs). Rabbit BMSCs-populated fibrin hydrogels with or without collagen were fabricated and cultured by the free floating method for 4 weeks. The DNA content of fibrin/collagen matrix significantly increased the growth of BMSCs compared to the fibrin-only matrix at 2week. Alkaline phosphatase activity was significantly higher in the fibrin/collagen matrix (71.0 nmol of p-nitrophenol /min/disc) than the fibrin-only matrix (45.1 nmol of p-nitrophenol /min/disc). Deposition of calcium was not significantly different between two groups. Histological examination also revealed more matured organization and deposition of collagen fibers and more concentric calcium deposition in the fibrin/collagen matrix compared to the fibrin-only matrix. These results indicated that fibrin/collagen matrix could be more effective than fibrin alone in supporting growth and osteogenic differentiation of BMSCs.



Key Engineering Materials (Volumes 288-289)

Edited by:

Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata




Y. I. Yang et al., "Effect of Fibrin/Collagen Matrix on Osteogenic Differentiation of Bone Marrow Stromal Cells", Key Engineering Materials, Vols. 288-289, pp. 35-38, 2005

Online since:

June 2005




[1] W. Bensaid, J.T. Triffitt, C. Blanchat, K. Oudina, L. Sedel, H. Petite. Biomaterials Vol. 24 (2003), pp.2497-2502.

DOI: https://doi.org/10.1016/s0142-9612(02)00618-x

[2] Y. Yamada, J.S. Boo, R. Ozawa, T. Nagasaka, Y. Okazaki, K. Hata, M. Ueda. J. Cranio-Maxill. Surg. Vol. 31 (2003), pp.27-33.

[3] C.M. Stirk, A. Reid, W.T. Melvin, and W.D. Thompson: Gen. Pharmacol. Vol. 35 (2000), pp.261-267 (e) (f) a) c) e) f) d) b).