Biomimetic Mineralization and Bioactivity of Phosphorylated Chitosan
Phosphorylated chitosans were synthesized as templates to manipulate hydroxyapatite (HA) crystal nucleation, growth and microstructure. Two kinds of insoluble phosphorylated chitosan were soaked in saturated Ca(OH)2 solution for 4 d and in 1.5× SBF (simulated body fluid) solutions for 14 d at 37 °C for biomimetic mineralization. A lower [P]-content of phosphorylated chitosan promoted greater mineralization than higher [P]-content. Phosphorylated chitosan inhibited osteoblast proliferation and differentiation in vitro, while calcium phosphate phosphorylated chitosan composites did not.
Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata
Z. Q. Chen et al., "Biomimetic Mineralization and Bioactivity of Phosphorylated Chitosan ", Key Engineering Materials, Vols. 288-289, pp. 429-432, 2005