Two-Dimensional Array Formation of Multi-Cellular Spheroids on Micro-Patterned Polymer Brush Surface
Here, a two-dimensional microarray of ten thousand (100x100) hepatocyte hetero-spheroids, underlaid with endothelial cells, was successfully constructed with a 100 µm spacing in an active area of 20x20 mm on micro-fabricated glass substrates coated with poly(ethylene glycol) (PEG) brushes (Fig. 1). Co-cultivation of hepatocytes with endothelial cells was essential to stabilize hepatocyte viability and liver-specific functions, allowing us to obtain hepatocyte spheroids with a diameter of 100 µm, functioning as a miniaturized liver to secret albumin for at least three weeks. The spheroid array constructed here is highly useful as a platform of TBB and CBB to detect a wide variety of clinically, pharmacologically, and toxicologically active compounds through a cellular physiological response.
Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata
H. Otsuka et al., "Two-Dimensional Array Formation of Multi-Cellular Spheroids on Micro-Patterned Polymer Brush Surface", Key Engineering Materials, Vols. 288-289, pp. 449-452, 2005