Research on Platelet Adsorption Behavior Using Enzyme Immunoassays and LDH Testing
Platelet adhesion and activation restrict the clinical applicability of blood-contacting biomaterial because platelet-biomaterial interaction can result in the formation of a haemostatic plug or thrombus. In this study we used LDH (lactate dehydrogenase) tests to evaluate the adsorption behavior of platelets on material surfaces. Enzyme immunoassay (EIA) was applied to evaluate platelet activation using a special monoclonal antibody directly binding to the Pselectin on the activated platelet membrane. The results show compared with the conventional detecting tools of platelet adhesion / activation such as optical microscopy. LDH (lactate dehydrogenase) testing and EIA (enzyme immunoassay) are surface-sensitive methods for the investigation of various aspects of platelet adsorption and activation on different biomaterials.
Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata
A. S. Zhao et al., "Research on Platelet Adsorption Behavior Using Enzyme Immunoassays and LDH Testing", Key Engineering Materials, Vols. 288-289, pp. 515-520, 2005