Research on Platelet Adsorption Behavior Using Enzyme Immunoassays and LDH Testing

Abstract:

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Platelet adhesion and activation restrict the clinical applicability of blood-contacting biomaterial because platelet-biomaterial interaction can result in the formation of a haemostatic plug or thrombus. In this study we used LDH (lactate dehydrogenase) tests to evaluate the adsorption behavior of platelets on material surfaces. Enzyme immunoassay (EIA) was applied to evaluate platelet activation using a special monoclonal antibody directly binding to the Pselectin on the activated platelet membrane. The results show compared with the conventional detecting tools of platelet adhesion / activation such as optical microscopy. LDH (lactate dehydrogenase) testing and EIA (enzyme immunoassay) are surface-sensitive methods for the investigation of various aspects of platelet adsorption and activation on different biomaterials.

Info:

Periodical:

Key Engineering Materials (Volumes 288-289)

Edited by:

Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata

Pages:

515-520

DOI:

10.4028/www.scientific.net/KEM.288-289.515

Citation:

A. S. Zhao et al., "Research on Platelet Adsorption Behavior Using Enzyme Immunoassays and LDH Testing", Key Engineering Materials, Vols. 288-289, pp. 515-520, 2005

Online since:

June 2005

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Price:

$35.00

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