Biomimetic Synthesis of Apatite - Polyelectrolyte Complex (Chitosan - Phosphorylated Chitosan) Hydrogel as an Osteoblast Carrier

Abstract:

Article Preview

A novel three-dimensional scaffold of hydroxyapatite(HA)-polyelectrolyte complex (PEC) composite hydrogel was synthesized by a biomimetic method. PEC hydrogel was formed from equal volumes of 1% phosphorylated chitosan in water and 1% chitosan in 1% acetic acid solution. This PEC hydrogel was soaked in saturated Ca(OH)2 solution for 4 d and then in accelerated calcification solution (ACS) for 7 d, both at 37 oC. The PEC hydrogel was a nano-composite material with multiple levels of hierarchical porosity; hydroxyapatite (HA) crystals nucleated and grew on the fiber surfaces of the hydrogel; Rat osteoblasts were then seeded in this three-dimensional scaffold of HA-PEC composite hydrogel, the three-dimensional scaffold of HA-PEC hydrogel revealed excellent biocompatibility.

Info:

Periodical:

Key Engineering Materials (Volumes 288-289)

Edited by:

Xingdong Zhang, Junzo Tanaka, Yaoting Yu and Yasuhiko Tabata

Pages:

75-78

Citation:

Q. L. Li et al., "Biomimetic Synthesis of Apatite - Polyelectrolyte Complex (Chitosan - Phosphorylated Chitosan) Hydrogel as an Osteoblast Carrier", Key Engineering Materials, Vols. 288-289, pp. 75-78, 2005

Online since:

June 2005

Export:

Price:

$38.00

[1] W Xian, J.X. Tang, P.A. Janmey, W.H. Braunlin. Biochemistry. 38 (1999), p.7219.

[2] S.I. Stupp, P.V. Braun. Science. 277 (1997), p.1242.

[3] S.I. Stupp, G.W. Ciegler. J Biomed Mater Res. 26 (1992), p.169.

[4] M Tanahashi, T Matsuda. J Biomed Mater Res. 34 (1997), p.305.

[5] C Du, F.Z. Cui, W Zhang, et al. J Biomed Mater Res. 50 (2000), p.518.

[6] K.S. TenHuisen, R.I. Martin, M Klimkiewicz, et al. J Biomed Mater Res. 29 (1995), p.803.

[7] J.D. Hartgerink , E Beniash, S.I. Stupp. Science. 294 (2001), p.1684.

[8] H.B. Wen, J.C. Wolke, J.R. deWijn, et al. Biomaterials. 18 (1997), p.1471.

[9] K Nishi, A Ebina, S Nishimura, et al. Int J Biol Macromol. 8 (1986), p.311.

[10] H Ohgushi, Y Dohi, T Katuda, et al. J Biomed Mater Res. 32 (1996), p.333.

[11] JW Lee, SY Kim, SS Kim, et al. J Appl Polym Sci. 73 (1999).