Cytotoxicity of Various Calcium Phosphate Ceramics
The cytotoxicity of five calcium phosphate ceramics, hydroxyapatite (HAp), flouroapatite (FAp), α-tricalcium phosphate (α-TCP), β-tricalcium phosphate (β-TCP) and tetracalcium phosphate (TTCP), was investigated. Based on the guidelines of biological test for medical devices in Japan, a cytotoxicity test of these calcium phosphates was carried out using Chinese hamster V79 lung fibroblasts. The cytotoxic study revealed that FAp and α-TCP showed high cytotoxicities. From various analyses, it was considered that the cytotoxicity of the FAp was due to fluorine ions extracted in a culture medium and the cytotoxicity of α-TCP resulted from a decrease in pH of the medium by the phosphoric acid, which produced by hydrolysis of( the α-TCP.
Takashi Nakamura, Kimihiro Yamashita and Masashi Neo
M. Tamai et al., "Cytotoxicity of Various Calcium Phosphate Ceramics", Key Engineering Materials, Vols. 309-311, pp. 263-266, 2006