Cytotoxicity of Various Calcium Phosphate Ceramics

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The cytotoxicity of five calcium phosphate ceramics, hydroxyapatite (HAp), flouroapatite (FAp), α-tricalcium phosphate (α-TCP), β-tricalcium phosphate (β-TCP) and tetracalcium phosphate (TTCP), was investigated. Based on the guidelines of biological test for medical devices in Japan, a cytotoxicity test of these calcium phosphates was carried out using Chinese hamster V79 lung fibroblasts. The cytotoxic study revealed that FAp and α-TCP showed high cytotoxicities. From various analyses, it was considered that the cytotoxicity of the FAp was due to fluorine ions extracted in a culture medium and the cytotoxicity of α-TCP resulted from a decrease in pH of the medium by the phosphoric acid, which produced by hydrolysis of( the α-TCP.

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Periodical:

Key Engineering Materials (Volumes 309-311)

Main Theme:

Edited by:

Takashi Nakamura, Kimihiro Yamashita and Masashi Neo

Pages:

263-266

DOI:

10.4028/www.scientific.net/KEM.309-311.263

Citation:

M. Tamai et al., "Cytotoxicity of Various Calcium Phosphate Ceramics", Key Engineering Materials, Vols. 309-311, pp. 263-266, 2006

Online since:

May 2006

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$38.00

[1] Y. Ito, N. Tanaka, Y. Fujimoto, Y. Yasunaga, O. Ishida, M. Agung and M. Ochi: J. Biomed. Mater. Res. Vol. 69A (2004), p.454.

[2] Y. Wang, T. Uemiura, J. Dong, J. Tanaka and T. Tateishi: Tissue Eng. Vol. 9 (2003), p.1205.

[3] S.M. Barinov, F. Rustichelli, P.V. Orlovskii, A. Lodini, S. Oscarsson, A.S. Firstov, V.S. Tumanov, P. Millet and A. Rosengren: J. Mater. Sci: Mater in Med. Vol. 15 (2004), p.291.

DOI: 10.1023/b:jmsm.0000015490.32304.f4

[4] K. Cheng, W. Weng, H. Qu, P. Du, G. Shen, G. Han, J. Yang and M.J. Ferreira: J. Biomed. Mater. Res. B Vol 69 (2004), p.33.

[5] E.L. Carey, H.H. Xu, G.C. Simon, S. Takagi and C.L. Chow: Biomaterials Vol 26 (2005), P. 5002.

[6] M.E. Ooms, J.G.C. Wolke, J.P.C.M. Waerden and J.A. Jansen: J. Biomed. Mater. Res. Vol. 61 (2002), p.9.

[7] M. Tamai, T. Isshiki, K. Nishio, M. Nakamura, A. Nakahira and H. Endoh: J. Mater. Res. Vol. 18 (2003), p.2633.

[8] T. Morita, T. Nagaki, I. Fukuda and K. Okumura: Muta. Res. Vol. 268 (1992).

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