Air-coupled ultrasound is a non-contact technique and has obvious advantages over water-coupled experimentation. Especially, wood materials are very sensitive to water and inspection without any coupling medium of a liquid is really needed to wood materials due to the permeation of coupling medium such as water. In this work, it is desirable to perform contact-less nondestructive evaluation to assess wood material homogeneity. A wood material was nondestructively characterized with non-contact and contact modes to measure ultrasonic velocity using automated data acquisition software. We have utilized a proposed peak-delay measurement method. Also through transmission mode was performed because of the main limitation for aircoupled transducers, which is the acoustic impedance mismatch between most materials and air. The variation of ultrasonic velocity was found to be consistent to some degree with those in infiltrated area for air-coupled and conventional scan images. Through-transmission scans for aircoupled and conventional UT were used based on both amplitude and time-of-flight images. Finally, results using a peak-delay measurement method well somewhat corresponded to ultrasonic velocities of the pulse overlap method.