Three-Dimensional Microfabrication System for Scaffolds in Tissue Engineering


Article Preview

Understanding chondrocyte behavior inside complex, three-dimensional environments with controlled patterning of geometrical factors would provide significant insights into the basic biology of tissue regenerations. One of the fundamental limitations in studying such behavior has been the inability to fabricate controlled 3D structures. To overcome this problem, we have developed a three-dimensional microfabrication system. This system allows fabrication of predesigned internal architectures and pore size by stacking up the photopolymerized materials. Photopolymer SL5180 was used as the 3D microfabricated scaffolds. The results demonstrate that controllable and reproducible inner-architecture can be fabricated. Chondrocytes from human nasal septum were cultured in 3D scaffolds for cell adhesion behavior. Such 3D scaffolds might provide effective key factors to study cell behavior in complex environments and could eventually lead to optimum design of scaffolds in various tissue regenerations such as cartilage, bone, etc. in a near future.



Key Engineering Materials (Volumes 326-328)

Edited by:

Soon-Bok Lee and Yun-Jae Kim






S. J. Lee et al., "Three-Dimensional Microfabrication System for Scaffolds in Tissue Engineering", Key Engineering Materials, Vols. 326-328, pp. 723-726, 2006

Online since:

December 2006




[1] R. P. Lanza, R. Langer, W. L. Chick, Principles of Tissue Engineering, Academic Press, New York(1997).

[2] Hutmacher DW., Scaffold design and fabrication technologies for engineering tissues state of the art and future perspectices, J Biomater Sci Polym Ed, 12(1), (2001), pp.107-124.

[3] I. H. Lee, D. -W. Cho, An Investigation on Photo-polymer Solidification Considering Laser Irradiation Energy in Micro-sterelithography, Microsystem Technologies, Vol. 10, No. 8-9, (2004), pp.592-598.

DOI: 10.1007/s00542-003-0337-4

[4] Gazell Mapili, Yi Lu, Shaochen Chen, Krishnendu Roy, Laser-layered microfabrication of spatially patterned functionalized tissue-engineering scaffolds, Journal of Biomedical Materials Research Part B: Applied Biomaterials, Vol. 75B, Issue 2, (2005).

DOI: 10.1002/jbm.b.30325

[5] Koji Ikuta, Akira Yamada, Fuminori Niikura, Real Three-dimensional Microfabrication for Biodegradable Polymers, Proceedings of the 26 th Annual International Conference of the IEEE EMBS, San Francisco, CA, USA, sep 1-5, (2004), pp.2679-2682.

DOI: 10.1115/imece2005-82154

[6] T. B. F. Woodfield, J. Malda, J. de Wijn, F. Peters, J. Riesle, C. A. van Blitterswijk, Design of porous scaffolds for cartilage tissue engineering using a three-dimensional fiber-deposition technique, Biomaterials 25 (2004), pp.4149-4161.

DOI: 10.1016/j.biomaterials.2003.10.056

In order to see related information, you need to Login.