The Effect of 3D Construction Culture of Human Chondrocytes Using Alginate Sponge
In this study, we investigated the effect of the use of alginate sponge as a chondrocyte-3D scaffold for the construction of a cartilage graft. Alginate sponge was made by 5% alginic acid which was crosslinked by CaCl2. Chondrocytes were obtained from a nasal septum after the operation and cultured in 3D alginate sponge. For analysis of cell differentiation, we have checked aggrecan, collagen type I and II using RT-PCR and performed the histological and scanning electron microscopy analysis. Our experiments showed that alginate sponge of 5% promoted sufficient chondrocyte proliferation and differentiation, resulting in the formation of a specific cartilage matrix. The sponge presents new perspectives with respect to in vitro production of "artificial" cartilage. We conclude that the alginate sponges have potential as a scaffold for cartilage tissue engineering.
Soon-Bok Lee and Yun-Jae Kim
J. S. Lee, B. Kim, M. S. Kim, S. J. Lee, S. W. Kim, D. W. Cho, J. S. Kim, G. B. Lim, "The Effect of 3D Construction Culture of Human Chondrocytes Using Alginate Sponge", Key Engineering Materials, Vols. 326-328, pp. 883-888, 2006