Novel Gene-Transferring System Using a Laminin-DNA-Apatite Composite Layer

Abstract:

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A laminin–DNA–apatite composite layer was successfully formed on the surface of an ethylene–vinyl alcohol copolymer. The immobilized DNA was transferred to the cells adhering onto the laminin–DNA–apatite composite layer more efficiently than those adhering onto a lamininfree DNA–apatite composite layer. It is considered that laminin immobilized in the surface layer enhances cell adhesion and spreading, and DNA locally released from the layer is effectively transferred into the adhering cells, taking advantage of the large contact area. The present gene transferring system, which shows high efficiency and safety, would be useful in gene therapy and tissue engineering.

Info:

Periodical:

Key Engineering Materials (Volumes 330-332)

Main Theme:

Edited by:

Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu

Pages:

1021-1025

DOI:

10.4028/www.scientific.net/KEM.330-332.1021

Citation:

A. Oyane et al., "Novel Gene-Transferring System Using a Laminin-DNA-Apatite Composite Layer", Key Engineering Materials, Vols. 330-332, pp. 1021-1025, 2007

Online since:

February 2007

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Price:

$35.00

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