Novel Gene-Transferring System Using a Laminin-DNA-Apatite Composite Layer
A laminin–DNA–apatite composite layer was successfully formed on the surface of an ethylene–vinyl alcohol copolymer. The immobilized DNA was transferred to the cells adhering onto the laminin–DNA–apatite composite layer more efficiently than those adhering onto a lamininfree DNA–apatite composite layer. It is considered that laminin immobilized in the surface layer enhances cell adhesion and spreading, and DNA locally released from the layer is effectively transferred into the adhering cells, taking advantage of the large contact area. The present gene transferring system, which shows high efficiency and safety, would be useful in gene therapy and tissue engineering.
Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu
A. Oyane et al., "Novel Gene-Transferring System Using a Laminin-DNA-Apatite Composite Layer", Key Engineering Materials, Vols. 330-332, pp. 1021-1025, 2007