Quantitative Analysis of Core Binding Factor 1 (Cbfa1) and Osteocalcin in C2C12 Cells Induced by Ca/P Ceramics In Vitro

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The mRNA expression of Cbfa1 and osteocalcin gene induced by calcium phosphate ceramics (Ca/P) were quantitative analyzed according to real-time RT-PCR method in this work. C2C12 cells were co-culture with four kinds of porous Ca/P ceramics for 2 and 5d without adding other growth factors. The four kinds of Ca/P ceramics were pure hydroxyapatite (HA) sintered at 1250°C and HA/TCP with a ratio of 60/40 sintered at 1100°C (HT1), 1200°C (HT2) and 1250°C (HT3) respectively. Real-time RT-PCR analysis found the Ca/P ceramics induced positive expression of Cbfa1 and osteocalcin in C2C12 cells, After 5 days culture, Cbfa1 and osteocalcin showed obvious higher expression compared with that in 2 days. Cbfa1 and osteocalcin expression in BCP was much higher than HA, and the expression level of osteocalcin was HT1>HT2>HT3>HA. Our results showed that Ca/P ceramics alone were sufficient to induce C2C12 cells to osteoblastic differentiation and the sinter temperature and phase composition of Ca/P ceramics could affect their osteoindctive capacity significantly.

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Periodical:

Key Engineering Materials (Volumes 330-332)

Main Theme:

Edited by:

Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu

Pages:

1067-1070

DOI:

10.4028/www.scientific.net/KEM.330-332.1067

Citation:

Y. F. Tan et al., "Quantitative Analysis of Core Binding Factor 1 (Cbfa1) and Osteocalcin in C2C12 Cells Induced by Ca/P Ceramics In Vitro", Key Engineering Materials, Vols. 330-332, pp. 1067-1070, 2007

Online since:

February 2007

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$35.00

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