Ectopic Osteogenesis of Bone Marrow Stromal Cells Loading in Hydroxyapatite/ β-Tricalcium Phosphate
This study was to determine the ectopic osteogenic ability of BMSCs in combination with a scaffolding material comprising hydroxyapatite and β-tricalcium phosphate matrix (HA/β-TCP). BMSCs were obtained from the SD rats and induced to osteogenesis. Then these induced cells were seeded into HA/β-TCP and the constructs were auto-implanted subcutaneously for up to 12 weeks. Histological analysis, immunostaing, RT-PCR and transmission electron microscopy of the retrieved specimens at various intervals showed obvious trends of ectopic bone formation with obvious alteration of cellular phenotype.
Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu
Y. F. Lin et al., "Ectopic Osteogenesis of Bone Marrow Stromal Cells Loading in Hydroxyapatite/ β-Tricalcium Phosphate", Key Engineering Materials, Vols. 330-332, pp. 1109-1112, 2007