Repairing Periodontal Bone Defect with In Vivo Tissue Engineering Bone


Article Preview

The purpose of this study was to develop a feasible approach for repairing periodontal bone defects with the in vivo tissue engineering bone incorporated with bioabsorbable PLA membrane and to provide evidences for the clinical application. Osteoinductive HA/β-TCP sintered at 1100°C were implanted in the femur medial muscles in the hind legs of three dogs. Four weeks after implantation, the in vivo tissue engineering (TE) bone was explanted. Meanwhile, artificial periodontal bone defects of 8mm×6mm were performed on the buccal side of 4th premolar and 1st molar of mandible bilaterally, with the exposure of dental roots. The defects were treated as follows: (1) in vivo TE bone and PLA membrane; (2) HA/β-TCP ceramics and PLA membrane; (3) PLA membrane only; (4) empty control. At the 2, 4, 8 weeks post-transplantation, the dogs were sacrificed. The specimen were harvested and evaluated by gross inspection, dental radiography, SPECT (99mTC-MDP) and histological observation by MPIA2500. The results showed that more mature osseointegration was found in the group 1. We presumed that the in vivo TE bone graft could enhance the reparation of periodontal bone defects.



Key Engineering Materials (Volumes 330-332)

Main Theme:

Edited by:

Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu




B. Zhang et al., "Repairing Periodontal Bone Defect with In Vivo Tissue Engineering Bone", Key Engineering Materials, Vols. 330-332, pp. 1121-1124, 2007

Online since:

February 2007




[1] Schnurer SM,Gopp U, Kuhn KD: Orthpade. Vol. 32 (2003), p.2.

[2] Linda G, Griffith, Gail Naughton: Science. Vol. 276(1997), p.181.

[3] X.D. Zhang, H.P. Yuan, K de Groot: Biomaterials with inteinsic Osteoinductive(The 6th World Biomaterials Congress, Hawaii, USA, 2000).

[4] C Bao, H Fan, C Deng, et al: Key Engineering Material Vol. 254-256(2004), p.801.

[5] Molly M. Stevens, Robert P. Marini , Dirk Schaefer , et al: PNAS, Vol. 102(32)(2005), p.11450.

[6] Robert F. Service: Science, Vol. 309(2005), p.683.

[7] Ashammakhi N, Mäkelä EA, Vihtonen K, et al. Cambridge Healthtech Institute's Advances in Orthopaedic Tissue Engineering. Washington, DC: Jan 24, (2000).

[8] Giardino R, Fini M, Nicoli Aldini N, et al: J Trauma Inj Infect Crit Care, 47(1999), p.303 A B C.