Repair of Articular Cartilage Defect with Cell-Loaded Nano-HA/PLGA Composites
A novel porous composite scaffold of nano-HA/poly (lactic-co-glycolic) (PLGA) was fabricated by solvent casting/particulate leaching method. Chondrocytes were isolated from the knee articular joints of a rabbit, and then seeded in the scaffolds. The cell-loaded scaffolds were cultured in vitro for 5 days before implantation. Full-thickness articular cartilage defects were created in rabbits, and filled with and without the cell-loaded nano-HA/PLGA scaffolds. The implants were harvested after in vivo incubation of 2 and 5 weeks. Cartilaginous tissues were observed at defects repaired with the cell-loaded scaffolds, while only fibrous tissues were found for the control groups. The repaired tissues were evaluated histologically by hematoxylin and eosin staining. Results revealed that nano-HA/PLGA composite scaffolds facilitated adheration of cells in vitro, and the nano-HA particles could prevented the scaffolds from collapsing and promoted the formation of cartilaginous tissue in vivo.
Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu
H. Lu et al., "Repair of Articular Cartilage Defect with Cell-Loaded Nano-HA/PLGA Composites", Key Engineering Materials, Vols. 330-332, pp. 1185-1188, 2007