Effect of Surface Electrostatic Property on Chondrogenic Differentiation of Human Mesenchymal Stem Cells

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In this study, three kinds of photo-reactive polymers, namely positively charged azidophenyl-derived polyallylamine (AzPhPAAm), negatively charged azidophenyl-derived poly(acrylic acid) (AzPhPAAc) and neutral azidophenyl-derived poly(ethylene glycol) (AzPhPEG), were synthesized by introduction of photo-reactive group (-N3) into polyallylamine, poly(acrylic acid) and poly(ethylene glycol), respectively. The structure of the azidophenyl-derived polymers was confirmed by 1H-NMR measurement. All of the photo-reactive polymers can be pattern-grafted on the surface of cells culture plate, which was confirmed by the optical microscopy observation. The contact angle decreased after surface modification by the photo-reactive polymers. Human mesenchymal stem cells (MSC) cultured on the modified surfaces showed different morphology. The cells adhered and spread more on the PAAm- and PAAc-grafted surfaces than on the PEG-grafted surface. The pellets formed on PAAm- and PEG-grafted surfaces in condrogenic differentiation medium were positively stained by safranin O/ fast green. But the pellet formed on PAAc-grafted surface did not show obviously positive staining for safranin O/ fast green. These results indicate that the PAAm- and PEG-grafted surfaces promoted chondrogenic differentiation of MSC.

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Periodical:

Key Engineering Materials (Volumes 330-332)

Main Theme:

Edited by:

Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu

Pages:

1189-1192

Citation:

L. Guo et al., "Effect of Surface Electrostatic Property on Chondrogenic Differentiation of Human Mesenchymal Stem Cells", Key Engineering Materials, Vols. 330-332, pp. 1189-1192, 2007

Online since:

February 2007

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$38.00

[1] Webb K, Hlady V, Tresco PA. J Biomed Mater Res vol. 41(1998) p.422.

[2] helton Rm, Rasmussen AC, Davies JE. Biomaterials vol. 9(1988)p.24.

[3] Van Wachem PB, Hogt AH, Van Aken WG Biomaterials vol. 8(1987) p.323 a b d c.

[4] Daw R, Candan S, Beck AJ. Biomaterials vol. 19(1988) p.1717.

[5] Bos R, Van der Mei HC, Busscher HJ. FEMS Microbiol vol23(1999) p.179.

[6] J Yang, YQ Wan, JL Yang, JZ Bei, SG Wang J Biomed Mater Res vol. 67A(2003)p.1139.

[7] Young Gun Ko, Young Ha Kim, Dong June Ahn etal Biomaterials vol. 22(2001) p.2115.

[8] GP Chen, Y Imanishi, Y Ito Langmuir vol. 14(1998) p.6610.

[9] Takehisa Matsuda, Takashi Sugawara Langmuir vol. 11(1995) p.2272.