Effect of Surface Electrostatic Property on Chondrogenic Differentiation of Human Mesenchymal Stem Cells
In this study, three kinds of photo-reactive polymers, namely positively charged azidophenyl-derived polyallylamine (AzPhPAAm), negatively charged azidophenyl-derived poly(acrylic acid) (AzPhPAAc) and neutral azidophenyl-derived poly(ethylene glycol) (AzPhPEG), were synthesized by introduction of photo-reactive group (-N3) into polyallylamine, poly(acrylic acid) and poly(ethylene glycol), respectively. The structure of the azidophenyl-derived polymers was confirmed by 1H-NMR measurement. All of the photo-reactive polymers can be pattern-grafted on the surface of cells culture plate, which was confirmed by the optical microscopy observation. The contact angle decreased after surface modification by the photo-reactive polymers. Human mesenchymal stem cells (MSC) cultured on the modified surfaces showed different morphology. The cells adhered and spread more on the PAAm- and PAAc-grafted surfaces than on the PEG-grafted surface. The pellets formed on PAAm- and PEG-grafted surfaces in condrogenic differentiation medium were positively stained by safranin O/ fast green. But the pellet formed on PAAc-grafted surface did not show obviously positive staining for safranin O/ fast green. These results indicate that the PAAm- and PEG-grafted surfaces promoted chondrogenic differentiation of MSC.
Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu
L. Guo et al., "Effect of Surface Electrostatic Property on Chondrogenic Differentiation of Human Mesenchymal Stem Cells", Key Engineering Materials, Vols. 330-332, pp. 1189-1192, 2007