Various reinforcements have been used to reinforce hydroxyapatite composites. Bioactivity of hydroxyapatite and excellent mechanical properties of titanium make titanium fiber reinforced hydroxyapatite composite a promising biomaterial. In this work, a titanium fiber was chosen as the reinforcement, and hydroxyapatite powders prepared by wet precipitation method were used as matrix material. The interface between titanium fiber and hydroxyapatite was investigated in terms of microstructure and chemical composition using a combination of scanning electron microscopy (SEM), X-ray diffraction (XRD) and energy disperse spectroscopy (EDS). Experimental results showed that titanium/hydroxyapatite composite with controllable interface could be made by choosing proper preparation method.