The Influence of Bovine Serum Albumin on the Biomimetic Calcium Phosphate Coating of Bioactivated Titanium
In this study, bovine serum albumin protein (BSA) was introduced to investigate the co-precipitation process of calcium phosphate and BSA on bioactivated Ti. Commercially pure titanium were bioactivated firstly, and then immersed in a highly supersaturated stable calcium phosphate (Ca-P) solution at three different conditions. The samples designated as Ti-C, Ti-C-CB, and Ti-C-B for control. The samples were evaluated by SEM with EDX, XRD and XPS. The co-precipitation of BSA protein and Ca-P influenced the morphology of the crystals of Ti-C-CB significantly. In terms of the immersion in the Ca-P solution containing BSA, the co-precipitation of Ca-P with BSA on the surface of Ti-C-CB was a chemical process rather than simple physical adsorption, which was most possibly achieved by the linkage of –COO− groups to Ca-P. Such coprecipitated interaction led to the formation of a tight, dense and uniform Ca-P coating.
Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu
Y. Wu et al., "The Influence of Bovine Serum Albumin on the Biomimetic Calcium Phosphate Coating of Bioactivated Titanium", Key Engineering Materials, Vols. 330-332, pp. 581-584, 2007