pDNA and Calcium Phosphate Co-Precipitation on Titanium Alloy Plate


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DNA transfection by biomaterial is a promising strategy to stimulate cells in tissue engineering. Co-precipitation of Calcium Phosphate (CaP) with plasmid DNA (pDNA) is known for several decades as non-viral transfection agent [1]. In this study, we report the co-precipitation of different pDNA into biomimetic calcium phosphate coating onto titanium alloy (Ti) plates in order to evaluate their efficiency of incorporation. And it is possible to tune the rate of coprecipitation by changing the volume of Calcium Phosphate Solution (CPS) and the [pDNA]. The structure of coating was affected by the presence of DNA in CPS solution.



Key Engineering Materials (Volumes 330-332)

Main Theme:

Edited by:

Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu




L. Yang et al., "pDNA and Calcium Phosphate Co-Precipitation on Titanium Alloy Plate", Key Engineering Materials, Vols. 330-332, pp. 621-624, 2007

Online since:

February 2007




[1] Sambrook J, Russell DW. Introducing cloned gene into cultured mammalian cells. In: Molecular cloning: A laboratory manual, 3rd ed. Cold Spring Harbor, NY: Cold Spring Harbor Lab; 2001. pp.1614-1619.

DOI: https://doi.org/10.1101/pdb.prot3186

[2] Barrere F, Layrolle P, van Blitterswijk CA, de Groot K. J. Mat. Sci. Mat. Med. Vol. 12 (2001), p.529.