Titanium oxide films were obtained by MAO at the applied voltages of 250-550V and their bio-mineralization behavior was investigated. The films were composed mainly of TiO2 phases in the form of anatase and rutile and enriched with Ca and P elements in the form of CaTiO3 and amorphous calcium phosphate. Their bio-mineralization behavior was evaluated in a simulated body fluid (SBF). After immersed in SBF for 72 h, white mineralized layers were observed on the samples obtained at high voltages. The bio-mineralized rate of samples increased with the applied voltages, which resulted in the difference on morphology of different samples. The structure and composition of the films have an important effect on their bio-mineralization behavior.