Immobilization of Collagen on Hydroxyapatite and Its Interation with Cells


Article Preview

The adhesion of bone cells on substrate materials is generally measured by the removal torque and/or contact area between bone and implants. In this study, collagen was used as a surfacegrafting material on hydroxyapatite (HA) substrates to enhance the cell adhesion because the collagen is a major constituent of connective tissues and has been regarded as one of the most excellent coating materials for bone bonding. First, HA disks (10mmΦ x 1mm) were prepared and then collagen was immobilbized on the HA surface using an 3-APTES coupling agent to improve the adhesiveness of cells on HA disk surfaces. NIH 3T3 fibroblasts were seeded on the collagengrafted and non-grated HA disks and cultured in a Dulbecco’s modified eagle medium containing 10% fetal bovine serum for 3 hrs to evaluate the cell adhesion on the HA samples. The fibroblasts on the collagen-grafted sample were more spread than those on the non-grafted sample. It is believed that collagen-grafted HA surface provides suitable sites for cell attaching due to the high biocompatibility of collagen.



Key Engineering Materials (Volumes 330-332)

Main Theme:

Edited by:

Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu




K.H. Jung et al., "Immobilization of Collagen on Hydroxyapatite and Its Interation with Cells", Key Engineering Materials, Vols. 330-332, pp. 781-784, 2007

Online since:

February 2007




[1] S. Weiner, H.D. Wagner, Annu. Rev. Mater. Sci. Vol. 28 (1998), p.271.

[2] S. Shi, M. Kirk, A.J. Kahn, J. Bone Miner. Res. Vol. 11 (1998), p.3938.

[3] M. Bigerelle, K. Anselme, J. Biomed. Res. Vol. (2005), p.530.

[4] D.A. Puleo, Biomaterials Vol. 17 (1996), p.217 (a) (b).