Covalent Immobilization of Heparin on Anatase TiO2 Films via Chemical Adsorbent Phosphoric Acid Interface
Heparin is covalently immobilized onto the surface of anatase TiO2 film using the bifunctional linking reagent, APTES (3-Aminopropyltriethoxylsilane), which can be bonded to the film by reaction between ethoxyl of the linking reagent and hydroxyl of the film. Compared to the control, the immobilization is enhanced by phosphoric acid chemical pre-adsorption on the film for there is more hydroxyl group present. Platelets deposited to heparinized surface showed only minor spreading and aggregation. The results of this study suggest that heparin immobilization to anatase TiO2 films via phosphoric acid interface may improve the in vivo blood compatibility.
Xingdong Zhang, Xudong Li, Hongsong Fan, Xuanyong Liu
Y. J. Weng et al., "Covalent Immobilization of Heparin on Anatase TiO2 Films via Chemical Adsorbent Phosphoric Acid Interface", Key Engineering Materials, Vols. 330-332, pp. 865-868, 2007